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干旱胁迫对不同藜麦种子萌发及生理特性的影响
引用本文:温日宇,刘建霞,张珍华,郭耀东,代旭瑶,姜庆国,樊丽生.干旱胁迫对不同藜麦种子萌发及生理特性的影响[J].作物杂志,2019,35(1):121-12.
作者姓名:温日宇  刘建霞  张珍华  郭耀东  代旭瑶  姜庆国  樊丽生
作者单位:1 山西省农业科学院玉米研究所,034000,山西忻州2 山西大同大学生命科学学院,037009,山西大同
基金项目:山西省重点研发计划项目(201603D221004-3);山西省重点研发计划项目(201603D221004-5);山西省农业科学院创新项目(YCX2017D2101);山西省农业科学院创新项目(YCX2018D2T04);大同市重点研发项目(2018042);大同大学大创项目(XDC2018233)
摘    要:为研究不同干旱程度对不同藜麦种子萌发及幼苗生长期耐旱性的影响,以忻藜1号(黑藜)、忻藜2号(白藜)和忻藜3号(红藜)为材料,采用培养皿萌发、土培育苗的方法,设置6个PEG 6000浓度梯度(0、5%、10%、15%、20%、25%),对3种藜麦的种子和幼苗进行处理,测定其种子发芽率、发芽势、下胚轴长、根长等指标和幼苗脯氨酸含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性等生理指标。结果表明:(1)随着PEG 6000浓度的升高,藜麦种子的发芽势、发芽率、下胚轴长和根长都呈现逐渐降低的趋势,用回归分析求得白藜半致死PEG 6000干旱胁迫浓度为20.02%,黑藜为20.25%,红藜为24.70%;(2)随着PEG 6000浓度的升高,3种藜麦幼苗SOD和POD活性都呈先升后降的趋势;MDA和脯氨酸含量与干旱胁迫程度呈显著正相关关系。由此可得,红藜耐旱性最强,黑藜次之,白藜最差。

关 键 词:黑藜  白藜  红藜  干旱胁迫  种子萌发  生理特性  
收稿时间:2018-08-07

Effects of Drought Stress on Germination and Physiological Characteristics of Different Quinoa Seeds
Riyu Wen,Jianxia Liu,Zhenhua Zhang,Yaodong Guo,Xuyao Dai,Qingguo Jiang,Lisheng Fan.Effects of Drought Stress on Germination and Physiological Characteristics of Different Quinoa Seeds[J].Crops,2019,35(1):121-12.
Authors:Riyu Wen  Jianxia Liu  Zhenhua Zhang  Yaodong Guo  Xuyao Dai  Qingguo Jiang  Lisheng Fan
Institution:1 Maize Research Institute, Shanxi Academy of Agricultural Sciences, Xinzhou 034000, Shanxi, China2 College of Life Science, Datong University, Datong 037009, Shanxi, China
Abstract:To study drought tolerance of different buckwheat seed germination and seedling growth stages under different drought conditions, the experiments were conducted on the basis of "Xinli No.1 (Black quinoa), Xinli No.2 (White quinoa), and Xinli No.3 (Red quinoa)". Using the method of germination and soil-cultivation in petri dishes, six PEG 6000 concentration gradients (0, 5%, 10%, 15%, 20%, 25%) were set to treat the seeds and seedlings of three types of quinoa. The growth indexes and physiological indicators of seedlings were determined. The results showed that: (1) With the increase of PEG 6000 concentration, the germination potential, germination rate, hypocotyl length and root length of quinoa seeds all showed a decreasing trend, and regression analysis was used to find the concentration of semi-lethal PEG 6000 drought stress. White quinoa was 20.02%, black quinoa was 20.25%, and red quinoa was 24.70%. (2) With the increase of PEG 6000 concentration, the activities of superoxide dismutase and peroxidase of the three buckwheat seedlings showed a trend of rising first and then decreasing. The contents of malondialdehyde (MDA) and proline were similar. The degree of drought stress showed a significant positive correlation to the contents of MDA and proline. This results showed the highest drought-tolerance in red quinoa, followed by black and white quinoa.
Keywords:Black quinoa  White quinoa  Red quinoa  Drought stress  Seed germination  Physiological characteristics  
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