The measurements of acid detergent fibre in rapeseed by visible and near-infrared spectroscopy |
| |
| Authors: | R Font B Wittkop A G Badani M Del Río-Celestino W Friedt W Lühs A De Haro-Bailón |
| |
| Institution: | Department of Agronomy and Plant Breeding, Institute of Sustainable Agriculture, CSIC, 14080 Cordoba, Spain;Department of Plant Breeding, IFZ, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany;C.I.F.A., Junta de Andalucia, Alameda del Obispo s/n, 14080, Cordoba, Spain |
| |
| Abstract: | Visible and near‐infrared spectroscopy (NIRS) calibrations for acid detergent fibre (ADF) in intact rapeseed (Brassica napus L.) were performed for two different sample volumes (10 ml, 500 seeds approximately; and 1 ml, 50 seeds approximately). The inclusion of brown and yellow‐seeded cultivars in this work has allowed the whole range of ADF currently described in the literature for this character to be covered. Chemometric techniques have been used for developing calibration equations for both procedures when measuring the two different seed sample volumes. On the basis of the coefficient of determination in the cross‐validation (R2cv) obtained for the 10 and 1 ml assays (0.80 and 0.73), and SECV/SEL ratios (2.30 and 2.57), respectively, both equations showed an accuracy sufficient for screening purposes in an ADF range from 6.80 to 13.46% dry wt, which is presented in this work. |
| |
| Keywords: | Brassica napus acid detergent fibre brown and yellow-seeded intact seed near-infrared spectroscopy two seed sample volumes |
|
|
