| 大兴安岭北方森林凋落物真菌及其抗菌化合物 |
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| 引用本文： | 张哲栋,梁晶,李泽宇,高思禹,邱天艺,单体江,徐利剑.大兴安岭北方森林凋落物真菌及其抗菌化合物[J].中国农学通报,2021,37(6):104-110. |
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| 作者姓名： | 张哲栋 梁晶 李泽宇 高思禹 邱天艺 单体江 徐利剑 |
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| 作者单位： | 1.黑龙江大学现代农业与生态环境学院,哈尔滨 150080;2.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091;3.华南农业大学林学与风景园林学院/广东省微生物信号与作物病害防控重点实验室,广州 510642 |
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| 基金项目： | 国家自然科学基金“大兴安岭林下凋落物慢速生长产孢真菌的隐秘代谢物及其抗菌活性”(31870528);黑龙江省博士后科研启动金“兰伯盘菌W17聚酮类代谢物抗水稻病原菌的研究”(LBH-Q15123);东北盐碱植被恢复与重建教育部重点实验室开放基金“水稻脂类代谢盐碱地胁迫响应关键基因及代谢途径网络构建”(20200522-4) |
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| 摘 要： | 为了研究大兴安岭森林凋落物真菌的抗菌活性,首先利用颗粒涂布平板法分离真菌,通过扩增与比对内部转录间隔区序列初步鉴定分离到的真菌。采用双平板打孔药剂扩散法检测抗菌活性,使用层析法分离目标菌株单体化合物。通过波谱学分析确定其化学结构,使用96孔板液体稀释法检测单体化合物的最低抑菌浓度。共分离得到88株真菌,其隶属于57个属、74个分类单元,其中18株真菌内部转录间隔区序列相似性≤97%。其中6株真菌的乙酸乙酯提取物具有抗菌活性,2株真菌KNFL008和KNFL040抗菌活性较强。经活性追踪分离,共得到4个单体化合物,分别为化合物A（1,3-2-羟基-5-十五烷基苯）、化合物B（麦角固醇）、化合物C（2-氨基-3H-苯氧嗪-3-酮）和化合物D（2-乙酰氨基-3H-苯氧嗪-3-酮）。对化合物A、B、C进行了最低抑菌浓度的测定,为3.13~200 μg/mL。化合物A和D为首次在真菌提取物中分离。本研究为进一步开发利用大兴安岭凋落物真菌资源打下了基础。
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| 关 键 词： | 大兴安岭 凋落物 真菌 抗菌活性 次级代谢产物 化合物分离 |
| 收稿时间： | 2020-05-28 |
Litter Fungi in the Boreal Forests and Their Antibacterial Compounds in the Greater Khingan Mountains |
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| Zhang Zhedong,Liang Jing,Li Zeyu,Gao Siyu,Qiu Tianyi,Shan Tijiang,Xu Lijian.Litter Fungi in the Boreal Forests and Their Antibacterial Compounds in the Greater Khingan Mountains[J].Chinese Agricultural Science Bulletin,2021,37(6):104-110. |
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| Authors: | Zhang Zhedong Liang Jing Li Zeyu Gao Siyu Qiu Tianyi Shan Tijiang Xu Lijian |
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| Institution: | 1.College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080;2.Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091;3.College of Forestry and Landscape Architecture, South China Agricultural University/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642 |
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| Abstract: | To study the antimicrobial activity of forest litter fungi in the Greater Khingan Mountains, firstly, fungi were isolated by a method of spreading particles on plates, and the isolated fungi were preliminarily identified by the amplification and alignment of internal transcribed spacer sequences. The antibacterial activities were detected by a two-layer-plate diffusion method and monomeric compounds of targeted strains were separated by chromatography. Spectroscopic analyses were used to determine the chemical structures of the monomeric compounds. A 96-well plate liquid dilution method was used to detect their minimum inhibitory concentration. A total of 88 fungi were isolated, belonging to 57 genera and 74 taxa. Of them, the internal transcribed spacer sequence similarities of 18 fungi were not higher than 97%. Among them, the ethyl acetate extracts of 6 fungi had antibacterial activities, and the two fungal strains KNFL008 and KNFL040 had stronger antibacterial activities than the others. By a bioassay-guided separation, four monomeric compounds, A (1,3-2-hydroxy-5-pentaalkylbenzene), B (ergosterol), C (2-amino-3h-phenoxyzin-3-ketone) and D (2-acetylamino-3h-phenoxyzin-3-ketone), were separated. The minimum inhibitory concentration of compound A, B and C was tested as 3.13-200 μg/mL. Compound A and D were firstly isolated from fungal extracts. This study lays a foundation for the further resource development and utilization of litter fungi in the Greater Khingan Mountains. |
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| Keywords: | the Greater Khingan Mountains litter fungus antibacterial activity secondary metabolites compound separation |
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