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| 章丘大葱子叶再生体系建立及再生株倍性鉴定 | |
| 引用本文： | 穆春华,刘 霞,于元杰,王金辉.章丘大葱子叶再生体系建立及再生株倍性鉴定[J].中国农学通报,2008,24(8):355-359. |
| 作者姓名： | 穆春华 刘 霞 于元杰 王金辉 |
| 作者单位： | 1. 山东省农业科学院玉米研究所,济南,250100 2. 山东农业大学农学院,泰安,271018 3. 聊城市东昌府区农业局技术站,聊城,252000 |
| 摘 要： | 以章丘大葱子叶为材料，研究了不同激素配比对愈伤组织诱导和继代、芽分化、芽点的伸长及根诱导的影响，并对获得的再生植株进行了鉴定。结果表明，诱导章丘大葱子叶产生愈伤组织、愈伤组织的继代培养、愈伤组织芽分化、芽点的伸长及诱导生根的最佳培养基及激素配比分别为：MS+2,4-D 2.0mg/L+KT 0.5mg/L、MS＋2,4-D 2.5mg/L＋KT 0.5mg/L、MS+2,4-D 0+KT 1.0mg/L、MS+KT 0.5mg/L和MS基本培养基；试管苗经驯化移栽后，共有22株成活，成活率为92%，对成活的再生植株进行形态学、叶片气孔及根尖染色体鉴定表明，共有3株发生变异，其中2株为外部形态上的变异，而另一株为染色体的加倍变异。 |
| 关 键 词： | 大葱 子叶 再生体系 再生植株 |
| 收稿时间： | 2008-05-04 |
| 修稿时间： | 2008-06-16 |
Constitute of Regeneration System of Spring Onion Via Cotyledon and Judge of Tetraploid Plantlets |
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| Mu Chunhu,Liu Xi,Yu Yuanjie,Wang Jinhui.Constitute of Regeneration System of Spring Onion Via Cotyledon and Judge of Tetraploid Plantlets[J].Chinese Agricultural Science Bulletin,2008,24(8):355-359. | |
| Authors: | Mu Chunhu Liu Xi Yu Yuanjie Wang Jinhui |
| Institution: | Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100; Department of Agronomy, Shandong Agricultural University, Tai’an 271018; Technical Institution, Agricultural Bureau of Dongchangfu, Liaocheng 252000 |
| Abstract: | Zhangqiu spring onion was used as experimental materials. We studied the optimum medium and hormone combination of callus induction from cotyledons, subculture of callus, shoot regeneration from callus, shoot-extension induction, root induction and identified the chromosome number of the plantlet. The results showed that: MS+2,4-D 2.0mg/L+KT 0.5mg/L, MS＋2,4-D 2.5mg/L＋KT 0.5mg/L, MS+2,4-D 0+KT 1.0mg/L, MS+KT 0.5 mg/L and basal MS medium. One plantlet among twenty two was determined as a tetraploidy based on the plant morphology, observation of the leaf stoma and chromosome number of the root, other two plantlets represented morphological variation. |
| Keywords: | spring onionzz cotyledonzz regeneration systemzz plantletzz |
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