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氨态氮、亚硝态氮、硝态氮对麦穗鱼的急性毒性研究
引用本文:潘坤,王卫民,祝东梅.氨态氮、亚硝态氮、硝态氮对麦穗鱼的急性毒性研究[J].中国农学通报,2012,28(35):96-101.
作者姓名:潘坤  王卫民  祝东梅
作者单位:1. 华中农业大学水产学院/农业动物遗传育种与繁殖教育部重点实验室,武汉430070;郧阳师范高等专科学校生物化学与环境工程系,湖北十堰442000
2. 华中农业大学水产学院/农业动物遗传育种与繁殖教育部重点实验室,武汉,430070
基金项目:国家水体污染控制与治理科技重大专项(水专项)中的"水环境监测的新技术、新方法研发与应用示范"
摘    要:为了对麦穗鱼的人工养殖和作为水生毒理学的实验材料提供参考。在水温(12.7±1)℃、溶氧5.60~9.87 mg/L的条件下,研究了氨态氮、亚硝态氮、硝态氮对麦穗鱼的急性毒性。试验结果表明:非离子氨对麦穗鱼的24、48、72、96 h的LC50值(95%置信区间)分别为0.435 mg/L (0.407~0.466 mg/L)、0.384 mg/L (0.356~0.417 mg/L)、0.371 mg/L (0.344~0.402 mg/L)、0.346 mg/L (0.316~0.383 mg/L),非离子氨对麦穗鱼的安全浓度是0.0346 mg/L。亚硝态氮对麦穗鱼的24、48、72、96 h的LC50值(95%置信区间)分别为422.862 mg/L (365.741~490.035 mg/L)、327.746 mg/L (276.875~394.054 mg/L)、266.291 mg/L (228.302~310.877 mg/L)、236.907 mg/L (200.488~281.108 mg/L),亚硝态氮对麦穗鱼的安全浓度是23.6907 mg/L。硝态氮对麦穗鱼的24、48、72、96 h的LC50值(95%置信区间)分别为2.155 g/L (2.074~2.242 g/L)、1.929 g/L (1.864~1.999 g/L)、1.769 g/L (1.701~1.842 g/L)、1.663 g/L (1.577~1.780 g/L),硝态氮对麦穗鱼的安全浓度是0.1663 g/L。在3种化合物中,非离子氨对麦穗鱼毒性最大,亚硝态氮次之,硝态氮对麦穗鱼的毒性最小。

关 键 词:三江    三江
收稿时间:9/5/2012 12:00:00 AM
修稿时间:2012/9/20 0:00:00

Studies on Acute Toxicity of Ammoniacal Nitrogen, Nitrite, and Nitrate for Pseudorasbora parva
Pan Kun , Wang Weimin , Zhu Dongmei.Studies on Acute Toxicity of Ammoniacal Nitrogen, Nitrite, and Nitrate for Pseudorasbora parva[J].Chinese Agricultural Science Bulletin,2012,28(35):96-101.
Authors:Pan Kun  Wang Weimin  Zhu Dongmei
Abstract:In order to provide reference for pseudorasbora parva artificial culture and as aquatic toxicology experimental material. The acute toxic effects of ammoniacal nitrogen, nitrite, and nitrate on pseudorasbora parva were studied at temperature (12.7±1)℃ and dissolved oxygen 5.60-9.87 mg/L. The test results showed that the 24 h-LC50,, 48 h-LC50, 72 h-LC50 and 96 h-LC50 (95 % confidence interval) of non-ionic ammonia to P. parva were 0.435 mg/L (0.407-0.466 mg/L), 0.384 mg/L (0.35-0.417 mg/L), 0.371 mg/L (0.344-0.402 mg/L), 0.346 mg/L (0.316-0.383 mg/L), respectively. The safety concentration of non-ionic ammonia to P. parva was 0.0346 mg/L. The 24 h-LC50, 48 h-LC50, 72 h-LC50 and 96 h-LC50 (95 % confidence interval) of nitrite to P. parva were 422.862 mg/L (365.74-490.035 mg/L), 327.746 mg/L (276.875-394.054 mg/L), 266.291 mg/L (228.302-310.877 mg/L), 236.907 mg/L (200.488-281.108 mg/L), separately. The safety concentration of nitrite to P. parva was 23.6907 mg/L. The 24 h-LC50, 48h-LC50, 72 h-LC50 and 96 h-LC50 (95 % confidence interval) of nitrate to P. parva were 2.155 g/L (2.074-2.242 g/L), 1.929 g/L (1.864-1.999 g/L), 1.769 g/L (1.701-1.842 g/L), 1.663 g/L (1.577-1.780 g/L), respectively. The safety concentration of nitrate to P. parva was 0.1663 g/L. In three kinds of compound, the toxicity of non-ionic ammonia to P. parva was maximum, nitrite was the second smallest, and the toxicity of nitrate to P. parva was minimum.
Keywords:

acute Toxicity

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