| 石油降解混合菌剂的筛选及降解条件研究 |
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| 引用本文： | 邱清华,哈尼帕,邓绍云,刘瑛,郝菲.石油降解混合菌剂的筛选及降解条件研究[J].中国农学通报,2013,29(3):184-189. |
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| 作者姓名： | 邱清华 哈尼帕 邓绍云 刘瑛 郝菲 |
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| 作者单位： | 伊犁师范学院,新疆奎屯833200 |
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| 基金项目： | 伊犁师范学院2011年度科研项目“石油降解菌株的筛选及降解条件优化研究”(2011KTYB011) |
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| 摘 要： | 为了获得石油高效降解混合菌剂及优化的降解条件，从油污土壤、含油废水及原油中经富集驯化分离得到细菌68株、真菌15株，放线菌6株；将所分离的菌株通过初筛、复筛及两两混合拮抗实验，筛选得到Y-4与Y-12及Y-4与Y-37两组石油高效降解混合菌剂，其对石油的降解率均超过60%。同时研究了不同的初始条件对各混合菌剂降解率的影响。结果表明，Y-4与Y-12组合在初始pH 8.0、转速160 r/min、种子液以1:1接种0.5 mL、30~35℃培养7天，Y-4与Y-37组合在初始pH 7.5、转速130~160 r/min、种子液以1:1接种0.5 mL、35℃培养7天，加入的初始原油量越少，基质营养越丰富，其降解率越高。通过实验可知，在适宜的条件下2种混合菌剂的降解率均较高，并且混合菌剂对石油的降解率要高于单菌剂。
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| 关 键 词： | 发酵滤液 发酵滤液 |
| 收稿时间： | 2012/4/16 0:00:00 |
| 修稿时间： | 2012/7/10 0:00:00 |
Study on the Screening and Degradation Condition of Oil Degradation Mixed Bacteria Agents |
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| Qiu Qinghua,Ha Nipa,Deng Shaoyun,Liu Ying,Hao Fei.Study on the Screening and Degradation Condition of Oil Degradation Mixed Bacteria Agents[J].Chinese Agricultural Science Bulletin,2013,29(3):184-189. |
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| Authors: | Qiu Qinghua Ha Nipa Deng Shaoyun Liu Ying Hao Fei |
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| Institution: | (Yili Normal University,Kuitun Xinjiang 833200) |
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| Abstract: | In order to obtain oil degrading mixed agents and optimized degradation conditions, sixty-eight bacteria, fifteen fungus and six actinomycetes were isolated from oily soil, oily wastewater, crude oil. Y-4 & Y-12 combination and Y-4 & Y-37 combination were selected through screening, rescreening and two-two mixed antagoinstic experiment, the oil degradation rates of the two mixed microbial agents were quite high by more than 60%. At the same time, the influence of different conditions on oil degradation of the mixed bacteria agents was studied. The results showed that, the degradation rates of the two mixed microbial agents, Y-4 & Y-12 combination in the initial pH 8.0, speed 160 r/min, the seed solution 0.5 mL with the ratio of 1:1, 30~ 35℃for 7 days, and Y-4 & Y-37 combination in the initial pH 7.5, speed 130-160 r/min, the seed solution 0.5 mL with the ratio of 1:1, 35℃for 7 days, were more higher with adding less initial crude oil volume and abundant matrix nutrition. The article experiment showed that, the oil degradation rates of the two mixed microbial agents were quite high under the appropriate conditions, and the degradation rate of the mixed microbial agents was higher than the single agent. |
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| Keywords: | petroleum mixed microbial agents selection degradation conditions |
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