| 陕西设施蔬菜根结线虫特异性分子检测 |
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| 引用本文： | 张锋,杨苗苗,孙娟,洪波.陕西设施蔬菜根结线虫特异性分子检测[J].中国农学通报,2014,30(31):136-140. |
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| 作者姓名： | 张锋 杨苗苗 孙娟 洪波 |
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| 作者单位： | 陕西省动物研究所,,陕西省动物研究所 |
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| 基金项目： | 国家科技支撑计划“丘陵沟壑区高效农业生产关键技术集成与示范”(2011BAD31B05-04)；陕西省科学院重大科技项目“大荔现代特色农业产业保障技术研究与示范”(2014K-03) |
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| 摘 要： | 为明确陕西设施蔬菜根结线虫的主要种类,利用已报道的花生根结线虫Meloidogyne arenaria（Neal.）Chitwood]、南方根结线虫Meloidogyne incognita（Kofold＆White）Chitwood]、北方根结线虫（Meloidogyne hapla Chitwood）和爪哇根结线虫Meloidogyne javanica（Treub）]的特异性引物,对陕西省20个县市区的24个样区的根结线虫进行PCR检测。样品中检测到了南方根结线虫、北方根结线虫、花生根结线虫和爪哇根结线虫,其中南方根结线虫为优势种群,大部分样区为单一种群为害,但个别样区存在2种种群。
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| 关 键 词： | 根结线虫 PCR检测 特异性引物 分子鉴定 |
| 收稿时间： | 2014/4/21 0:00:00 |
| 修稿时间： | 2014/4/21 0:00:00 |
Specific Molecular Assay for the Detection of Greenhouse Vegetables Meloidogyne spp. in Shaanxi |
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| Institution: | Zhang Feng, Yang Miaomiao, Sun Juan, Hong Bo, Zhang Shulian (1 Shaanxi Institute of Zoology, Xi＇ an 710032; 2Shananxi Xueqian Normal University, Xi＇ an 710100; 3Agricultural Technique Center of Fuping County, Fuping Shaanxi 711700) |
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| Abstract: | In order to identify the species of vegetable root-knot nematodes in Shaanxi Province,the reported specific primers of Meloidogyne arenaria（Neal.） Chitwood, Meloidogyne incognita（Kofold White） Chitwood,Meloidogyne hapla Chitwood and Meloidogyne javanica（Treub） were served as polymerase chain reaction （PCR） assay primers respectively to detect Meloidogyne spp. of greenhouse vegetables that obtained from twenty four sampling areas that distributed in twenty cities and counties in Shaanxi. Our study showed that M.arenaria, M. incognita, M. hapla and M. javanica were detected, and M. incognita was the dominant species;most sampling areas were infected by single population, however, specific sampling areas were infected by two populations. |
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| Keywords: | Meloidogyne spp PCR assay Specific primers Molecular identification |
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