|
|||||
|
|
| 不同浓度赖氨酸对猪IEC碱性氨基酸转运载体mRNA表达的影响 | |
| 引用本文： | 职爱民,左建军,周响艳,邹仕庚,黄志毅,王小兰,叶慧,代发文,冯定远.不同浓度赖氨酸对猪IEC碱性氨基酸转运载体mRNA表达的影响[J].中国农学通报,2010,26(21):6-11. |
| 作者姓名： | 职爱民 左建军 周响艳 邹仕庚 黄志毅 王小兰 叶慧 代发文 冯定远 |
| 作者单位： | 1. 华南农业大学动物科学学院,广州,510642;河南省农业科学院农业部动物免疫学重点开放试验室,郑州,450002 2. 华南农业大学动物科学学院,广州,510642 3. 福建海新集团,福建厦门,363102 |
| 基金项目： | 科技部;国家重点基础研究发展计划项目，国家基金委;广东联合基金项目 |
| 摘 要： | 本研究通过建立猪肠粘膜上皮细胞（Intestinal epithelial cells, IEC）原代培养的方法，研究了赖氨酸对体外培养的猪小肠粘膜上皮细胞碱性氨基酸转运载体mRNA表达的影响。用机械刮取初生仔猪小肠粘膜加胶原酶Ⅱ消化的方法，成功地在体外培养了新生仔猪IEC；分别用浓度为0.5 mM、2 mM、8 mM赖氨酸处理细胞96 h，提取细胞总RNA，采用实时荧光定量PCR方法，检测了碱性氨基酸转运载体在不同处理细胞中的表达丰度。结果表明：高浓度的赖氨酸对CAT-1 mRNA的表达有上调作用，差异极显著(P<0.01)，并且呈剂量依赖效应；不同浓度的赖氨酸对CAT-2 mRNA的表达影响不大，8 mM和2 mM赖氨酸组CAT-2 mRNA的表达丰度均有低于0.5 mM的趋势，但二者之间差异不显著(P>0.05)；不同浓度的赖氨酸对y+LAT1 mRNA表达影响差异不显著(P>0.05)；高浓度的赖氨酸可提高4F2hc mRNA的表达；0.5 mM和2 mM赖氨酸组4F2hc mRNA的表达丰度均低于8 mM，且差异显著(P<0.05)；0.5 mM和2 mM赖氨酸组之间差异不显著(P>0.05)。 |
| 关 键 词： | 朱砂根 朱砂根 RAPD 遗传多样性 聚类分析 |
| 收稿时间： | 2010/2/24 0:00:00 |
| 修稿时间： | 6/2/2010 12:00:00 AM |
The Influence of Different Lysine Concentration on the Cationic Amino Acid Transporter mRNA Expression of Porcine IEC |
|
| Zhi Aimin,Zuo Jianjun,Zhou Xiangyan,Zou Shigeng,Huang Zhiyi,Wang Xiaolan,Ye Hui,Dai Fawen,Feng Dingyuan.The Influence of Different Lysine Concentration on the Cationic Amino Acid Transporter mRNA Expression of Porcine IEC[J].Chinese Agricultural Science Bulletin,2010,26(21):6-11. | |
| Authors: | Zhi Aimin Zuo Jianjun Zhou Xiangyan Zou Shigeng Huang Zhiyi Wang Xiaolan Ye Hui Dai Fawen Feng Dingyuan |
| Institution: | Zhi aimin12 Zuo Jianjun Zhou Xiangyan3 Zou Shigeng Huang Zhiyi Wang Xiaolan 1,Ye Hui 1,Dai Fawen 1,Feng Dingyuan 1 (1 College of Animal Science,South China Agricultural University,Guangzhou 510642,2 Key Laboratory for Animal Immunology of the Ministry of Agriculture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,3 Fujian Haixin Group Co.Ltd,Xiamen Fujian 363102) |
| Abstract: | The influence of different lysine concentration on the cationic amino acid transporter mRNA expression porcine intestinal epithelial cells （IEC） in vitro was studied by the primary culture of porcine IEC.The primary culture of porcine IEC system was established by mechanical and enzymatic （collagenase Ⅱ） methods using small intestines removed from colostrum-deprived newborn piglets.Total RNA was isolated from IEC samples after treatment with 0,2 and 8 mmol/L lysine for 96 hours.The cationic amino acid transporters mRNA expression abundances were determined by real-time Q-PCR.The results were as follows：high concentrations of lysine had significant up-regulation effect on the CAT-1 mRNA expression and there was an obvious dose-dependent effect （P0.01）.Lysine treatment had no distinct effect on the CAT-2 mRNAexpression abundance.CAT-2 mRNA expressions of 8 mmol/L and 2 mmol/L lysine treatment were lower than that of 0 mmol/L lysine group,but with no significant difference （P0.05）.Lysine treatment had no significant effect on the y ＋ LAT1 mRNA expression abundance （P0.05）.High concentrations of lysine could enhance 4F2hc mRNA expression.4F2hc mRNA expression abundances of 0 mmol/L and 2 mmol/L lysine groups were significantly lower than that of 8 mmol/L lysine group （P0.05）.There was no significant difference between 0 mmol/L and 2 mmol/L group （P0.05）. |
| Keywords: | pig cationic amino acid transporter mRNA expression lysine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农学通报》浏览原始摘要信息 | |
| 点击此处可从《中国农学通报》下载免费的PDF全文 | |