| 马齿苋总黄酮的超声波辅助提取工艺优化及其抗氧化、抗肿瘤活性研究 |
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| 引用本文： | 梁艳妮.马齿苋总黄酮的超声波辅助提取工艺优化及其抗氧化、抗肿瘤活性研究[J].中国农学通报,2019,35(4):130-135. |
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| 作者姓名： | 梁艳妮 |
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| 作者单位： | 1.陕西中医药大学/陕西省中药资源产业化协同创新中心 |
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| 基金项目： | 陕西省科技厅重点产业创新链（群）项目“基于活性导向策略的寒性中药中抗肿瘤活性成分发现与评价研究”(2018ZDCXL-SF-01-02-02)； 陕西省科技厅项目“色胺酮治疗溃疡性结肠炎作用机制研究”(2018SF-193)。 |
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| 摘 要： | 优化马齿苋中总黄酮的甲醇浸提-超声波辅助提取最佳工艺，并评价其体外抗氧化、抗肿瘤活性。以甲醇浓度、提取温度、料液比和超声功率为因素，以总黄酮提取率为指标，通过单因素试验与正交设计优选马齿苋总黄酮的超声波辅助提取条件；测定其对DPPH?和?OH的清除能力评价抗氧化活性；MTT法测定其对人肺癌细胞A549体外增殖的影响。结果表明：马齿苋总黄酮的最佳提取工艺为：料液比1:50，温度80℃，超声功率200 W，甲醇浓度90%，提取30 min，提取2次，总黄酮提取率为6.37%。马齿苋总黄酮对DPPH?清除率在4.0 mg/mL为89.80%，对?OH清除率在20.0 mg/mL为86.07%；其质量浓度为420 μg/mL时对细胞A549增殖的抑制率为74.62%，与阳性药组无显著性差异。该优化工艺准确可靠，提取率高；马齿苋总黄酮具有较强的抗氧化和抗肿瘤活性。
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| 关 键 词： | 马齿苋 总黄酮 正交设计 DPPH. .OH A549 |
| 收稿时间： | 2018/9/12 0:00:00 |
| 修稿时间： | 2019/1/2 0:00:00 |
Optimization of Extracting Total Flavonoids from Portulaca oleracea L. by Ultrasonic Method and Evaluation of Their Antioxidant and Antitumor Activities in Vitro |
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| Abstract: | To explore the optimum extraction process of flavonoids from Portulaca oleracea L. by methanol leaching-ultrasonic assistance and evaluate its antioxidant and anti-cancer activities. Based on single factor experiment, methanol concentration, extraction temperature, solid- liquid ratio and ultrasonic power were enrolled in this experiment, and the extracting ratio of total flavonoids was used to evaluate the extraction process. The extraction process of total flavonoids from P. oleracea L. was optimized by orthogonal experiment. Meanwhile, the antioxidant activity of flavonoids was assessed by scavenging assays of DPPH· and ·OH. The viability of human lung cell line A549 was detected by MTT assay. The optimal extraction process was as follows: liquid-solid ratio of 1:50, water bath temperature of 80℃, ultrasonic power of 200 W, 90% methanol as extraction solvent, extraction time of 30 min, extraction of 2 times. The extraction rate of total flavonoids from P. oleracea L. was 6.37% under the optimal condition. The scavenging rate of DPPH· was 89.80% at 4.0 mg/mL, while the scavenging rate of ·OH was 86.07% at 20 mg/mL. The inhibition rate of cell A549 proliferation was 74.62% at 420 μg/mL, which had no significant difference from that of the positive drug group. The flavonoids from P. oleracea L. are extracted efficiently by the above optimized technology and the total flavonoids exhibit antioxidant and antitumor activities. |
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| Keywords: | Portulaca oleracea L flavonoids orthogonal design DPPH OH A549 |
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