| 利用RAPD、ISSR标记分析国兰种质资源遗传多样性的研究 |
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| 引用本文： | 李丽辉,胡 瑶,李宏告,张跃龙,张 勇,吴宏燕.利用RAPD、ISSR标记分析国兰种质资源遗传多样性的研究[J].中国农学通报,2018,34(29):42-47. |
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| 作者姓名： | 李丽辉 胡 瑶 李宏告 张跃龙 张 勇 吴宏燕 |
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| 作者单位： | 湖南省核农学与航天育种研究所,湖南省核农学与航天育种研究所,湖南省核农学与航天育种研究所,湖南省核农学与航天育种研究所,湖南省核农学与航天育种研究所,湖南红艳兰花园艺有限公司 |
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| 基金项目： | 湖南省科技厅重点研发计划项目“湖南兰花资源创新与产业开发研究”(2016NK2180)；湖南省农业科学院科技创新项目“特色花卉新资源 创制与轻简化栽培技术研究”(2017JC55)；湖南省农业生物辐照工程技术研究中心开放基金项目“湖南兰花新品种选育及扩繁技术研究” (2016KF005)。 |
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| 摘 要： | 旨在分析不同地区国兰间的亲缘关系，为国兰资源的开发及新品种的选育提供分子水平的参考依据。利用RAPD和ISSR分子标记技术，对7种国兰的21个品种资源进行遗传多样性和亲缘关系分析。结果表明：39个RAPD和ISSR引物在供试材料中共扩增出96条带型清晰的谱带，其遗传中31条为多态性条带；通过UPGMA聚类分析表明，21个国兰品种资源间遗传距离在1.91~6.60之间，其中春兰资源的遗传距离在1.91~4.38之间，建兰资源的遗传距离在2.60~6.20之间，寒兰资源的遗传距离在2.20~5.80之间，墨兰资源的遗传距离在3.52~6.60之间，表现出了较高的遗传多样性。聚类分析结果与传统的形态学分类结果基本一致，也说明分子标记可以在分子水平反映遗传资源的遗传多样性，具有灵敏度高、结果真实可靠等优点。本研究结果显示国兰品种间的亲缘关系与地理位置分布相关，为兰属植物的分类及遗传多样性研究提供了一定的理论支撑。
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| 关 键 词： | 国兰 RAPD ISSR 聚类分析 遗传多样性 |
| 收稿时间： | 2017/10/24 0:00:00 |
| 修稿时间： | 2018/9/17 0:00:00 |
Study on Genetic Diversity of China Orchid Resources Using RAPD and ISSR Markers |
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| Abstract: | The research aims at analyzing genetic relationship among China orchid in different areas, and providing reference for the resources exploitation and new variety breeding of China orchid at the molecular level .With RAPD and ISSR markers, the genetic diversity and genetic relationship of 21 cultivars of 7 species in China orchid were analyzed. The results showed that 96 clear bands were amplified with 39 RAPD and ISSR primers, 31 of these were polymorphic strips. UPGMA cluster analysis showed that the genetic distance ranged from 1.91 to 6.60 among 21 China orchid cultivars, the genetic distance of Cymbidium. goeringii was 1.91- 4.38, the genetic distance of C. ensifolium was 2.60- 6.20, the genetic distance of C. kanran Makino was between 2.20 and 5.80, the genetic distance of C. sinense was between 3.52 and 6.60, which showed higher genetic diversity. The results of cluster analysis were basically consistent with the traditional morphological classification results, indicating that genetic markers could reflect genetic resources diversity at the molecular level, with the advantages of high sensitivity and reliable results. It is concluded that there are some
correlations between genetic relationship and geographical location among China orchid varieties and the study provide certain theory support for Cymbidium classification and genetic diversity research. |
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| Keywords: | China orchid ISSR RAPD cluster analysis genetic diversity |
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