| 大叶南五味子多溶剂分步提取物的抗菌活性研究 |
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| 引用本文： | 蒋红,王占林,王宏军,周铁忠,景雪.大叶南五味子多溶剂分步提取物的抗菌活性研究[J].中国农学通报,2012,28(26):55-58. |
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| 作者姓名： | 蒋红 王占林 王宏军 周铁忠 景雪 |
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| 作者单位： | 1. 辽宁医学院,辽宁锦州,121001
2. 朝阳市动物疫病预防控制中心,辽宁朝阳,122000 |
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| 基金项目： | 辽宁省教育厅资助项目“加减银翘散中药制剂的药效学研究”（L2010256）; 辽宁省“百千万人才工程”培养经费资助项目“猪主要疫病综合防制配套技术研究推广”（2010921043） |
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| 摘 要： | 为了探讨大叶南五味子果实的体外抗菌活性。将大叶南五味子依次以石油醚、乙酸乙酯、无水乙醇，水为溶媒，每种溶剂均先用超声波提取20 min，再加热回流提取2 h，采用微管-平板法测定4种溶剂的提取物对鸡大肠杆菌、猪链球菌和金黄色葡萄球菌的最小抑菌浓度(MIC)。结果显示：有机溶媒提取率在0.35%~5.50%，水提取率达到51.2%；有机溶媒提取物对鸡大肠杆菌、猪链球菌、金黄色葡萄球菌的MIC为0.44~34.08 mg/mL，水提取物对鸡大肠杆菌、猪链球菌、金黄色葡萄球菌的MIC分别为204.63、329.13、232.5 mg/mL。说明大叶南五味子果实有机溶媒提取物虽然得率少，但其化学成分具有较高的抗菌活性。该研究为大叶南五味子临床应用、抗菌活性成分的分离奠定基础。
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| 关 键 词： | 产量构成因素 产量构成因素 |
| 收稿时间： | 1/9/2012 12:00:00 AM |
| 修稿时间： | 2012/2/29 0:00:00 |
Antibacterial Activity of Different Solvent Separate-step Extract from Kadsura marmorata in Vitro |
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| Jiang Hong
,
Wang Zhanlin
,
Wang Hongjun
,
Zhou Tiezhong
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Jing Xue.Antibacterial Activity of Different Solvent Separate-step Extract from Kadsura marmorata in Vitro[J].Chinese Agricultural Science Bulletin,2012,28(26):55-58. |
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| Authors: | Jiang Hong Wang Zhanlin Wang Hongjun Zhou Tiezhong Jing Xue |
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| Institution: | 1 Liaoning Medical University,Jinzhou Liaoning 121001;2 Chaoyang City Centers for Animal Disease Control and Prevention,Chaoyang Liaoning 122000) |
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| Abstract: | In order to explore antibacterial activity of Kadsura marmorata fruit in vitro.Kadsura marmorata which solved in petroleum ether,ethyl acetate,anhydrous ethanol and water in turn,was extracted 20 min by ultrasonic extraction,then 2 h with a hot reflux,respectively.The minimal inhibitory concentration（MIC） of four extracts was detected against chicken E.coli,Streptococcus Suis and Staphylococcus aureus with the microtubule-plate method.The results showed that extraction rates of organic solvent were 0.35%-5.50% and water was 51.2%.MIC of organic solvent extracts reached 0.44-34.08 mg/mL against chicken E.coli,Streptococcus Suis and Staphylococcus aureus,while MIC of water extract was 204.63 mg/mL,329.13 mg/mL,232.5 mg/mL against chicken E.coli,Streptococcus Suis and Staphylococcus aureus respectively.This study indicated that the Kadsura marmorata fruit extraction of organic solvent had lower yield,but higher antibacterial activity elements than extraction of water.This study laid the foundation for clinical application and separation of antibacterial activity components from Kadsura marmorata. |
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| Keywords: | Kadsura marmorata extract antibacterial activity chicken E coli Streptococcus Suis |
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