| 华南鳞盖蕨组织培养与假植技术研究 |
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| 引用本文： | 郭文杰,蔡宣梅,方少忠,林 真,鲁雪华.华南鳞盖蕨组织培养与假植技术研究[J].中国农学通报,2007,23(1):50-050. |
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| 作者姓名： | 郭文杰 蔡宣梅 方少忠 林 真 鲁雪华 |
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| 作者单位： | 福建省农业科学院生物技术研究所,福州,350003 |
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| 摘 要： | 试验进行了华南鳞盖蕨孢子果的萌发试验，并利用幼嫩茎段进行诱导愈伤组织; 愈伤组织的诱导与增殖培养基为MS＋6-BA2 mg/L+NAA0.2 mg/L;其芽分化最佳激素组合为MS+6-BA 0.4 mg/L;生根培养中MS基本培养基比1/2MS效果好，MS基本培养基不论附加生长素IBA、NAA或IBA＋NAA，均能有效地诱导植株生根，生根率高；假植以珍珠岩和泥炭土的混合物为基质效果优于珍珠岩、菜园土和河沙，具有通气、保水、营养等优点，假植成活率高，苗势健壮。
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| 关 键 词： | 过氧化氢酶 过氧化氢酶 酶结构 生理功能 |
| 修稿时间： | 2006-10-302006-11-09 |
Studies on Tissue Culture and Primary Nursery Transplanting of Cephalotaxus |
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| Guo Wenjie,Cai Xuanmei,Fang Shaozhong,Lin Zhen,Lu Xuehua.Studies on Tissue Culture and Primary Nursery Transplanting of Cephalotaxus[J].Chinese Agricultural Science Bulletin,2007,23(1):50-050. |
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| Authors: | Guo Wenjie Cai Xuanmei Fang Shaozhong Lin Zhen Lu Xuehua |
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| Institution: | Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003 |
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| Abstract: | The germination test of Sporocarps of Microlepia hancei prantl had been done,the callus can be induced from the delicate stem of Microlepia hancei prantl.The inducement and proliferation medium of callus was MS 6-BA2 mg/L NAA0.2 mg/L.The optimal combination of hormones of buds differentiation was MS 6-BA 0.4 mg/L.The effect of MS medium in radication culture was better than 1/2MS,the roots of plants were all efficiently induced in MS medium without reference to adding IBA,NAA or IBA NAA,the rate of radication was high.The effect of seedling cultured in the mixture of perlite and turf soil was better than perlite,garden soil and river sands.The mixture of perlite and turf soil has the excellences such as ventilate,water conservation,nourishment and so on.The survival rate was higher and seedling substance was also hale. |
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| Keywords: | Cephalotaxus Tissue culture Primary nursery transplanting |
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