| 3种长江珍稀鱼类精子超低温冷冻保存的初步研究 |
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| 引用本文： | 王明华,钟立强,陈友明,丁淑燕,秦 钦,边文冀,蔡永祥,陈校辉.3种长江珍稀鱼类精子超低温冷冻保存的初步研究[J].中国农学通报,2015,31(5):55-58. |
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| 作者姓名： | 王明华 钟立强 陈友明 丁淑燕 秦 钦 边文冀 蔡永祥 陈校辉 |
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| 作者单位： | (;1.江苏省淡水水产研究所,南京 210017；;2.江苏省海洋水产研究所,江苏南通 226007） |
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| 基金项目： | 基金项目：国家星火计划项目“长江洄游珍稀鱼类产业化关键技术集成”（2012GA690001）。 |
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| 摘 要： | 试验对西伯利亚鲟、美洲鲥鱼和暗纹东方鲀3种长江珍稀鱼类的精液进行超低温冷冻保存技术研究。以解冻后精子的活力为参数，探讨了稀释液、不同种类不同体积分数的保护剂、以及不同的精液稀释比例对实验鱼类精液进行超低温冷冻保存的保护效果。结果显示，3种长江珍稀鱼类以配方2和体积分数为10%甲醇组成抗冻保护剂，西伯利亚鲟精液按1:2稀释倍数，保存10天后检查精子活力，存活率可达(45±5.00)%。暗纹东方鲀精液按1:2稀释倍数，保存20天后检查精子活力，存活率可达(60.5±2.69)%。美洲鲥鱼精液按1:3稀释倍数，保存60天后检查精子活力，存活率可达(80.3±2.58)%。3种珍稀鱼类的精子保存以美洲鲥鱼效果最好。保存方法均采用三步法，在液氮面上方6 cm处平衡10 min，接着在液氮面上平衡5 min，最后投入液氮中冷冻保存。研究结果为保护长江珍稀鱼类的种质资源提供了理论基础。
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| 关 键 词： | 花生 花生 断根 植株生长 开花 |
| 收稿时间： | 2014/9/11 0:00:00 |
| 修稿时间： | 2/2/2015 12:00:00 AM |
Study on Sperm Cryopreservation of Three Rare Migratory Fishes in the Yangtze River |
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| Institution: | Bian Wenji;1., Cai Yongxiang;2., Chen Xiaohui;1.（;1.Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017;2.Institute of Oceanology & marine Fisheries, Nantong jiangsu 226007) |
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| Abstract: | The technique of sperm cryopreservation of three rare migratory fishes in the Yangtze River was studied, including Acipenser baerii, Alosa sapidissima and Takifugu obscurus. The success of sperm cryopreservation was evaluated by post-thaw sperm motility. To explore methods for sperm cryopreservation, this study evaluated the effects of different extenders, cryoprotectants, their volume fractions, equilibration time and dilution ratios. The result showed that the best sperm cryopreservation of motility of Acipenser baerii was (45±5.00)% and Takifugu obscurus (60.5±2.69)% were obtained by following procedure: the ringer solution and the semen were mixed at ratio of 2:1 in volume and added 10% methanol, while the best sperm cryopreservation of motility of Alosa sapidissima (80.3±2.58)% was obtained by following procedure: the ringer solution and the semen were mixed at ratio of 3:1 in volume and added 10% methanol. Sperms were cryopreserved by three-step cooling progam. These results provided the methods to conserve genetic resources in three rare migratory fishes in the Yangtze River. |
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| Keywords: | Acipenser baerii Alosa sapidissima Takifugu obscurus sperm cryopreservation |
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