| 猪源肠球菌两种致病基因和表型的检测 |
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| 引用本文： | 刘磊,崔保安,王亚宾,程金平,王中明,郭敏.猪源肠球菌两种致病基因和表型的检测[J].中国农学通报,2009,25(1):17-20. |
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| 作者姓名： | 刘磊 崔保安 王亚宾 程金平 王中明 郭敏 |
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| 作者单位： | 河南农业大学牧医工程学院兽医微生物实验室,郑州,450002 |
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| 摘 要： | 摘要：目的：调查53株猪源肠球菌2种致病基因和2种表型的分布。方法：应用聚合酶链反应检测53株肠球菌的2种致病基因,用50mL/L兔血平板和30g/L明胶平板检测β溶血和明胶溶解表型。结果：粪肠球菌、屎肠球菌和鸟肠球菌2种致病基因的检出率分别为cylA 94.4%、96.9%、100%; gelE 100%、87.8%、50%；β溶血检出率分别为72.2%、42.4%、0;明胶溶解检出率分别为100%、0、0。结论：细胞溶解素激活基因(cylA)明胶酶基因(gelE)为猪源肠球菌的主要致病基因;粪肠球菌2种致病基因和2种表型的检出率高于屎肠球菌;来源于新鲜猪肉中的粪肠球菌存在较强的毒力，提示注意食品卫生安全；在新鲜猪肉中检测到2株鸟肠球菌，基因型和表型检测说明其存在较弱毒力。
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| 关 键 词： | 猪 肠球菌 基因 溶血 明胶 |
| 收稿时间： | 2008-09-24 |
| 修稿时间： | 2008-11-17 |
Detection of two virulence determinants and phenotypes of Swine- derived enterococci |
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| Liu Lei,Wang Yabin,Cheng Jinping,Wang Zhongming,Guo Min,Cui Baoan.Detection of two virulence determinants and phenotypes of Swine- derived enterococci[J].Chinese Agricultural Science Bulletin,2009,25(1):17-20. |
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| Authors: | Liu Lei Wang Yabin Cheng Jinping Wang Zhongming Guo Min Cui Baoan |
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| Institution: | (The College of Animal Husbamdry And Veterinary, ttenan Agricultural University, Zhengzhou 450002) |
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| Abstract: | Abstract: Objective：To investigate the incidence of two virulence determinants and two phenotypes in 53 strains of enterococci derived Swine. Methods：PCR were used to detect the two virulence determinants in 53 isolates . The two phenotypes , including β-hemolysis and gelatin hydrolyze,were performed on agar plate containing 50mL/L rabbit blood and agar plate supplemented with 30g/L gelatin respectively. Results：In E.faecalis, E.faecium and E.avium, the rates of detection of the two virulence determinants were cylA 94.4%、96.9%、100%, gelE 100%、87.87%、50% , the β -hemolysis was 72.2%、42.4%、0 , the gelatin hydrolyze was 100%、0、0. Conclusions：cylA and gelE are the main virulence determinants in enterococcui derived swine. E. faecalis possess more virulence determinants and phenotypes than E. faecium. E. faecalis derived fresh pork possess stronger virlence determinants indicate we must pay attention to food sanitation. 2 strains E.avium were detected in fresh pork and E.avium possess low virulence by detection of Phenotype and Genotype. |
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| Keywords: | swine Enterococcus Genes Cytolysin Gelatin |
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