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益生菌芽孢杆菌的分离与初步鉴定
引用本文:赵士豪,杨会琴,赵春海.益生菌芽孢杆菌的分离与初步鉴定[J].中国农学通报,2012,28(18):276-281.
作者姓名:赵士豪  杨会琴  赵春海
作者单位:1. 河北经贸大学生物科学与工程学院,石家庄,050061
2. 滨州职业学院,山东滨州,256603
基金项目:河北省科技支撑项目“葡萄皮渣中生物活性物质高效提取关键技术”(10231016)
摘    要:鉴于抗生素在饲料添加剂中的滥用,研究高效复合益生菌来替代抗生素已变得越来越重要。益生菌是一类新型饲料(食品)添加剂,可改善人畜肠道环境,减少体内有害菌群及有害物质含量。在本实验中,利用大肠杆菌(Escherichia coli)为抑菌对象,分离到4株益生菌,实验证明其抑菌效果理想,经过经形态学观察及传统生理生化实验分析表明:本实验所获得的4株菌均为芽孢杆菌(Bacillus spp.),为进一步开发高效复合益生菌奠定菌种基础。

关 键 词:遗传分析  遗传分析  
收稿时间:2/9/2012 12:00:00 AM
修稿时间:2012/3/14 0:00:00

solation and Primary Identification of Bacillus for Probiotics
Zhao Shihao , Yang Huiqin , Zhao Chunhai.solation and Primary Identification of Bacillus for Probiotics[J].Chinese Agricultural Science Bulletin,2012,28(18):276-281.
Authors:Zhao Shihao  Yang Huiqin  Zhao Chunhai
Institution:1 College of Biology Sciense and Engineering,Hebei University of Economics and Business,Shijiazhuang 050061;2 Binzhou Polytechnic,Binzhou Shangdong 256603)
Abstract:In view of the abuse of antibiotics in feed additives,efficient composite probiotics would be used to replace antibiotics which had became more and more important.Probiotics was a novel feed(food) additive for mankind or animals,which could improve the intestinal environment,decrease the quantity of harmful microflora and the hazardous substances in the body.In this paper,Escherichia coli was used for probiotics isolation,and then four strains were obtained.The strains were identified by morphology observation and traditional physiology biochemistry experiments,and the results showed that they were all belonging to Bacillus spp.in the work.It would made basis for the use of probiotics.
Keywords:probiotics  isolation  identification  Bacillus spp  
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