Morphological characterisation and agronomic evaluation of transgenic broccoli (Brassica oleracea L. var. italica) containing an antisense ACC oxidase gene |
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| Authors: | Maria X Henzi Mary C Christey David L McNeil |
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| Institution: | (1) New Zealand Institute for Crop & Food Research Limited, Private Bag 4704, Christchurch, New Zealand;(2) Soil, Plant and Ecological Sciences Division, Lincoln University, PO Box 84, Canterbury, New Zealand |
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| Abstract: | Morphological characterisation and agronomic evaluation was conducted on 12 transgenic broccolilines containing a tomato antisense1-aminocyclopropane-1-carboxylic
acid (ACC) oxidase gene. Plants of three cultivars: Shogun (Sh), Green Beauty (Gy) and Dominator (D), were regenerated from
hairy root cultures after co-cultivation with Agrobacterium rhizogenes strain A4T harbouring the binary vector pLN35. The T-DNA of pLN35 contains genes encoding a tomato antisense ACC oxidase
gene (35S-ACC-5′7′) and a neomycin phosphotransferase II gene (NOS-NPTII-NOS) for kanamycin resistance. The transgenic plants
were transferred to a greenhouse and fertile plants obtained. Integration of the foreign DNA into the broccoli genome was
confirmed by the polymerase chain reaction and Southern analyses. Transgenic plants showed evidence of hairy root (HR)-induced
morphological changes to varying degrees. Of the 12 characterised transgenic lines, three lines(Gy/7, D/1 and D/2) performed
within the limits of acceptability for all head quality parameters analysed (size, density, colour, shape and leafiness).
The ethylene production from stalks of four field-grown transgenic lines of Green Beauty broccoli showed significant reductions
in activity relative to the control 98 h after harvest. The Dominator transgenic lines D/1 and D/2 showed significant improvements
in head colour relative to the control from 48 h after harvest. These results are consistent with the ethylene production
patterns determined previously for these lines. The head colour results are consistent with previous results suggesting that
two enzyme systems may be involved in broccoli senescence, giving two bursts of ethylene production, with only the second
burst inhibited by the antisense ACC oxidase gene used.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | 1-aminocyclopropane-1-carboxylic acid (ACC) ACC oxidase antisense gene Brassica oleracea ethylene |
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