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The induction in vitro of adventitious shoots in Rosa
Authors:Davina Lloyd  Andrew V Roberts  Keith C Short
Institution:(1) Department of Biology and Biochemistry, North East London Polytechnic, E15 4LZ London, England;(2) Department of Life Sciences, Trent Polytechnic, Clifton Lane, NG11 8NS Nottingham, England
Abstract:Summary Adventitious shoots were formed on excised leaves, roots and callus of Rosa persica x xanthina and on excised leaves of R. laevigata and R. wichuraiana on culture media that included BAP and NAA as growth regulators. Shoots formed freely on freshly cultured callus of R. persica x xanthina but their production declined in successive cultures and ceased after twelve weeks. Transplantation to soil was improved by rooting plantlets in cellulose plugs in vitro and transferring plantlets to soil while still in the plugs.
Keywords:Rosa persica  Rosa xanthina  Rosa laevigata  Rosa wichuraiana  Rosa rugosa  Rosa hybrida  rose  adventitious shoots  starch grains  transplantation  cellulose plugs  rose breeding
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