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Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.)
Authors:MA Chowdhury  Bert Vandenberg  T Warkentin
Institution:(1) Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada, Harrow, Ontario, N0R 1G0, Canada;(2) Crop Development Centre, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada
Abstract:Twenty two RAPD and 22 ISSR markers were evaluated for their potential use in determination of genetic relationships in chickpea (Cicer arietinum L.) cultivars and breeding lines. We were able to identify six chickpea cultivars/breeding lines by cultivar-specific markers. All of the cultivars tested displayed a different phenotype generated either by the RAPD or ISSR primers. Though ISSR primers generated less markers than RAPD primers, the ISSR primers produced higher levels of polymorphism (% of polymorphic markers per primer) than RAPD primers. A high level of within cultivar homogeneity was observed in chickpea. Cultivars/breeding lines originating from a common genetic background showed closer genetic relationship. Chickpea lines with similar seed type(kabuli or desi) had a tendency to cluster together. This revised version was published online in August 2006 with corrections to the Cover Date.
Keywords:chickpea            Cicer arietinum L    cultivar  ISSR  RAPD  variation
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