Isolation of a new repetitive DNA sequence from Secale africanum enables targeting of Secale chromatin in wheat background |
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| Authors: | Cheng Liu Zu-Jun Yang Guang-Rong Li Zi-Xian Zeng Yong Zhang Jian-Ping Zhou Zhao-Hui Liu Zheng-Long Ren |
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| Institution: | (1) School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, P.R. China;(2) Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Ya’an city, 625014, Sichuan, P.R. China;(3) Industrial Crop Breeding and Cultivation Institute, Sichuan Academy of Agriculture Sciences, Jianyang, 641400, Sichuan, P.R. China |
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| Abstract: | A genome specific DNA sequence that detects Secale africanum chromatin incorporated into wheat was developed in this study. Random amplified polymorphic DNA (RAPD) analysis was used
to search for genome specific DNA sequences of S. africanum in lines, R111, “mianyang11” (MY11) and wheat-rye 1RS/1BL translocations R25 and R57. A high copy rye-specific DNA segment
pSaD15940 of the S. africanum genome was obtained. The sequence of pSaD15 did not show any significant homology to other reported sequences in databases
and it is therefore a new repetitive sequence of Secale. PCR primers were designed for pSaD15940, which amplify a clear 887 bp fragment in S. africanum but not in any wheat. The primers also amplified an 887 bp fragment in other accessions of rye, Chinese Spring-Imperial rye
chromosome additions and a diverse range of material carrying different rye chromosomes or chromosomal segments. In situ hybridization
showed that probe pSaD15940 was specifically hybridized throughout all rye chromosomes arms except for the terminal regions. The advantage of the rye-specific
probe developed herein compared to those of previous reports is that it has been shown to be widely applicable to other Secale species. The probe will be useful as a molecular marker for the introgression of S. africanum and other rye chromosome segments into the wheat genome. |
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| Keywords: | Secale africanum Rye Molecular marker FISH Chromatin detection |
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