Genetic analysis of in vitro wheat somatic embryogenesis |
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| Authors: | J De Buyser J L Marcotte Y Henry |
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| Institution: | (1) Laboratoire de Génétique Végétale, CNRS, URA 115, Université de Paris XI, 91405 Orsay, France;(2) Laboratoire de Biologie Moléculaire Végétale, BT 430, URA CNRS 1128, Université Paris XI, 91405 Orsay, France |
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| Abstract: | Summary A spring wheat genotype which produces somatic embryos in vitro, after short and long-term culture, was tested for its ability to sexually transmit this embryogenic trait. Reciprocal crosses were performed between a embryogenic line and a nonembryogenic variety.Immature embryos were cultured on Murashige and Skoog medium plus 2 mg/l 2,4-dichlorophenoxyacetic acid, gelled with 5.5 g/l agarose. Somatic embryogenesis was not expressed in the F1's. In contrast, from several hundred immature embryos of the F2 generation of one cross, 10.7% and 1.6% expressed somatic embryogenesis in short and long-term cultures respectively. These percentages of embryogenic: non-embryogenic fits a model of a few complementary genes. The embryogenic capacity of the F2 genotypes depends on the presence of recessive alleles at these gene loci. The long-term wheat somatic embryogenesis capacity requires a more complex mechanism than the short-term one.Abbreviations CS
Chinese Spring
- Aq
Aquila
- E
Embryogenic
- NE
Nonembryogenic
- SC
Subculture |
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| Keywords: | Triticum aestivum somatic embryogenesis wheat tissue culture genetics |
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