Analysis of Chinese Spring regenerants obtained from short- and long-term wheat somatic embryogenesis |
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| Authors: | Y Symillides Y Henry J De Buyser |
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| Institution: | (1) Laboratoire de Génétique Végétale, Bt 360, URA CNRS 115, Université Paris XI, 91405 Orsay, France;(2) Plant Breeding and Biometry, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece;(3) Laboratoire de Biologie Moléculaire Végétale, Bt 430, URA CNRS 1128, Université Paris XI, 91405 Orsay, France |
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| Abstract: | Summary Somatic embryogenesis was initiated from  immature embryos on Murashige-Skoog (MS) medium plus 2 mg.l-1 2,4-dichlorophenoxyacetic acid, 2% sucrose and 0.6% agarose. Somatic embryos were isolated and regenerated into whole green plants on MS medium devoid of 2,4-D. These regenerants were previously demonstrated to differ in their mitochondrial DNA organization. In order to estimate their characteristics three progenies of short-term culture regenerants and three progenies of long-term culture regenerants were analyzed and compared to the parental line. These somaclones obtained from the wheat variety Chinese Spring were evaluated for variation of 13 agronomic and morphological quantitative characters in comparison to the parental line. Significant variation was observed for plant height, spike length, main tiller diameter, between the somaclones regenerated from long-term culture and their parent. Differences were observed to increase with the duration of culture, leading to a significant modification of the structure of the plants. Several changes occurred during the somatic tissue cultures, but to a lesser extent than has previously been described in the literature. |
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| Keywords: | somaclonal variation somatic embryogenesis tissue culture wheat Triticum aestivum |
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