| 基因组原位杂交技术在植物研究中的应用 |
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| 引用本文： | 关鹤,赵泓,云兴福,刘凡.基因组原位杂交技术在植物研究中的应用[J].分子植物育种,2006,4(Z1):99-105. |
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| 作者姓名： | 关鹤 赵泓 云兴福 刘凡 |
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| 作者单位： | 内蒙古农业大学，北京市农林科学院蔬菜研究中心 北京，100089，北京市农林科学院蔬菜研究中心，内蒙古农业大学，北京市农林科学院蔬菜研究中心，呼和浩特，010018，北京，100089，呼和浩特，010018，北京，100089 |
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| 基金项目： | 北京市科技新星计划项目 |
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| 摘 要： | 基因组原位杂交是以亲本之一的总基因组DNA做探针,另一亲本的基因组DNA做封阻,在荧光原位杂交技术的基础上发展起来的一种染色体/染色质检测技术。在其发展的十几年里,已在植物的基因组研究中发挥了重要的作用。应用这一技术可对多倍体中基因组之间的亲缘关系、基因组组成及起源进行研究;对杂交种中染色体组的组成进行分析;对代换系、附加系和易位系进行有效的鉴定,并对其中的外源染色体或染色体片段的来源、大小、数目及发生位点进行检测和定位。此外,利用基因组原位杂交技术还有助于确定物种间的同源性;研究杂交种中来源不同的染色质在核中的分布;探索B染色体的起源、染色体间的配对、重组、交换等现象。随着基因组荧光原位杂交技术体系的不断发展、完善和改进,其应用范围不断拓展,在植物基因组研究领域中发挥了越来越重要的作用。
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| 关 键 词： | 基因组原位杂交(GISH) 荧光原位杂交(FISH) 植物 基因组 |
Application of GISH in Plants Genomic Research |
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| Guan He, Zhao Hong Yun Xingfu Liu Fan Inner Mongolia Agricultural University,Huhhot, Beijing Academy of Agriculture and Forestry Science Vegetable Research Center.Application of GISH in Plants Genomic Research[J].Molecular Plant Breeding,2006,4(Z1):99-105. |
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| Authors: | Guan He Zhao Hong Yun Xingfu Liu Fan Inner Mongolia Agricultural University Huhhot Beijing Academy of Agriculture and Forestry Science Vegetable Research Center |
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| Institution: | Guan He1,2 Zhao Hong 2* Yun Xingfu1 Liu Fan2 1 Inner Mongolia Agricultural University,Huhhot,010018,2 Beijing Academy of Agriculture and Forestry Science Vegetable Research Center,100089 |
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| Abstract: | Genomic in situ hybridization (GISH), a modification of flourescence in situ hybridization (FISH), uses total genomic DNA from one parent as the labeled probe and the other parental genomic DNA as blocking DNA. It has played an important role in genomic research of plants in recent years. It can be used to study the relationship, component and origin of the genome in polyploidy; to discriminate the different ancestral genome donors in hybrids; to identify the translocation, substitution and addition line; to detect and reveal the origin, size, amount, and location of the alien chromosome or chromosome segments. Moreover, it is also used to disclose the relationship among different species and analyze the location of allo-chromatins in nuclei. It can be used to analyze the origin of B chromosome and to examine the homoeologous pairing, recombination and interaction in many plants. With the continuing development and improvement of the technical system, GISH has broadened its application field and played more and more important role in plant genomic research. |
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| Keywords: | Genomic in situ hybridization (GISH) Flourescence in situ hybridization (FISH) Plant Genome |
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