| 禾谷镰孢菌FGSG＿09482基因功能初探 |
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| 引用本文： | 李丹,侯占铭.禾谷镰孢菌FGSG＿09482基因功能初探[J].分子植物育种,2020(1):118-124. |
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| 作者姓名： | 李丹 侯占铭 |
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| 作者单位： | 内蒙古师范大学生命科学与技术学院 |
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| 基金项目： | 国家自然科学基金项目(31160280);内蒙古自治区自然科学基金项目(2015MS0311)共同资助 |
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| 摘 要： | 为研究禾谷镰孢菌FGSG09482基因的生物学功能,以禾谷镰孢菌为试材,采用Split-Marker基因敲除技术构建基因敲除盒,PEG (聚乙二醇)介导转化法转化禾谷镰孢菌原生质体,在含有潮霉素的培养基上培养筛选,获得FGSG09482基因敲除突变株。通过观察比较其敲除突变体表型,从而分析鉴定该基因的生物学功能。结果表明:以野生型PH-1作为对照,敲除突变体的菌落形态没有明显变化,分生孢子形态没有明显差异,培养滤液颜色没有明显区别。分生孢子侵染番茄果实实验表明,以番茄果实为侵染宿主,对比野生型PH-1,突变体致病力没有减弱。但突变体菌落的生长速率滞后于野生型PH-1;敲除突变体的产孢量低于野生型PH-1;有性杂交实验表明,敲除突变体几乎不产生子囊壳,野生型PH-1可以。本研究初步表明,FGSG09482基因对禾谷镰孢菌菌落的生长速率有一定的调控作用;对禾谷镰孢菌的分生孢子的产量、子囊壳的产生也有影响,推测FGSG09482基因可能与禾谷镰孢菌有性生殖、无性生殖过程相关。
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| 关 键 词： | 禾谷镰孢菌 FGSG09482 Split-Marker 基因敲除 |
Research on the Function of FGSG＿09482 Gene in Fusarium graminearum |
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| Li Dan,Hou Zhanming.Research on the Function of FGSG＿09482 Gene in Fusarium graminearum[J].Molecular Plant Breeding,2020(1):118-124. |
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| Authors: | Li Dan Hou Zhanming |
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| Institution: | (College of Life Science and Technology,Inner Mongolia Normal University,Hohhot,010020) |
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| Abstract: | In order to study the function of FGSG09482 gene in Fusarium graminearum, using Fusarium graminearum as test material, the Split-Marker strategy was applied for constructing the deletion cassettes of the FGSG09482 gene. The cassettes were transformed into the protoplasts of the wild type PH-1 mediated by the PEG method. The knockout mutants were selected on the medium containing hygromycin. By knocking out FGSG09482 gene of Fusarium graminearum, the aim of the research is to identify the biological function of the gene through observing the changes of phenotype of the deletion mutant. The results show: Compared with the wild type PH-1, the morphology of the colony and conidia and the color of the culture filtrate solution of the deletion mutant had no obvious changes. The infection assay also showed that the infection spot size on the fruit of the tomato had no difference between the mutant and wide type. However, the growth rate and the number of the conidia yield of mutant strains reduced significantly compared with the wild type PH-1. The sexual crossing experiment showed that the knockout mutant almost did not produce the perithecia while the wild type PH-1 could. The results suggested that the FGSG09482 gene regulated the mycelial growth of Fusarium graminearum. It may be related the sexual and asexual process and affected the production of the conidium and perithecia of the Fusarium graminearum. |
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| Keywords: | Fusarium graminearum FGSG09482 Split-Marker Gene knockout |
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