| 棉花耐盐性的SSR鉴定研究 |
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| 引用本文： | 张丽娜,叶武威,王俊娟,樊保香.棉花耐盐性的SSR鉴定研究[J].分子植物育种,2010,8(5). |
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| 作者姓名： | 张丽娜 叶武威 王俊娟 樊保香 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,安阳,455000 |
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| 基金项目： | 转基因生物新品种培育科技重大专项，"十一五"国家科技支撑计划 |
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| 摘 要： | 土壤盐碱化现已成为危害农业发展和生态环境的全球性问题,培育和鉴选棉花耐盐品种是合理开发利用盐碱地的有效途径。本研究选用25份耐盐(包括耐和抗)和23份盐敏感棉花种质为实验材料,采用SSR技术,展开了棉花耐盐性鉴定技术的有关研究。从DNA快速提取到PCR扩增和产物检测以及多标记组合鉴定等环节进行分析探讨,初步制定了一套适于棉花耐盐性分子鉴定的方法,即多标记组合鉴定法。并用11份材料对该方法进行了验证,结果表明和0.4%盐量胁迫法的鉴定结果的相符率达90.91%。初步研究结果表明多标记组合鉴定法可用于棉花耐盐分子标记辅助鉴定。
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| 关 键 词： | 棉花 耐盐 SSR标记组合 |
Identification of Salinity-tolerance on Cotton by SSR Markers |
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| Zhang Lina,Ye Wuwei,Wang Junjuan,Fan Baoxiang.Identification of Salinity-tolerance on Cotton by SSR Markers[J].Molecular Plant Breeding,2010,8(5). |
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| Authors: | Zhang Lina Ye Wuwei Wang Junjuan Fan Baoxiang |
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| Institution: | Zhang Lina Ye Wuwei Wang Junjuan FanBaoxiang Cotton Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Anyang,455000 |
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| Abstract: | Soil salinization has become a serious global problem affecting the agricultural development and the ecological environment.It is an effective way to farm saline land and to enhance the sustainable agricultural development by developing,identifying and screening the salinity tolerant varieties of cotton.25 salinity tolerant and 23 salinity sensitive cotton accessions were used in this study to identify salinity tolerance of cotton by SSR markers.A new set of preliminary methods system,called SSR multi-marke... |
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| Keywords: | Cotton Salinity tolerance SSR Multi-markers |
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