| 槟榔中槟榔碱体外抑菌活性的研究 |
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| 引用本文： | 罗士数,张海德,刘小玲,朱莉.槟榔中槟榔碱体外抑菌活性的研究[J].农产品加工．学刊,2010(9). |
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| 作者姓名： | 罗士数 张海德 刘小玲 朱莉 |
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| 作者单位： | 海南大学食品学院; |
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| 基金项目： | “十一五”国家科技支撑计划项目(2007BAD76B03); 海南大学科研基金项目(Rndy200501,hd09xm83) |
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| 摘 要： | 采用超声波提取法、乙醇回流提取法、超临界CO2萃取法获得了槟榔碱,以大肠杆菌、金黄色葡萄球菌、变形杆菌、枯草芽孢杆菌、白色念珠菌、炭疽芽孢杆菌、黑曲霉、青霉等8种菌作为供试菌,通过抗菌实验探讨槟榔碱的体外抗菌作用。实验结果表明,3种方法提取的槟榔碱对变形杆菌、白色念珠菌、炭疽芽孢杆菌有很好的抑制作用。超声波提取物的最低抑菌质量浓度(MIC)为2.6mg/mL;乙醇回流提取物的最低抑菌质量浓度(MIC)为1.4mg/mL;超临界CO2萃取物的最低抑菌质量浓度(MIC)为0.8mg/mL。对黑曲霉、青霉没有抑制作用。3种方法提取的槟榔碱的抑菌活性大小依次为:超临界CO2萃取法>乙醇回流提取法>超声波提取法。
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| 关 键 词： | 超声波提取法 乙醇回流提取法 超临界CO2萃取法 槟榔碱 抑菌作用 |
Study on Antimicrobial Activity of Arecoline from Betel Nut in Vitro |
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| Luo Shishu,Zhang Haide,Liu Xiaoling,Zhu Li.Study on Antimicrobial Activity of Arecoline from Betel Nut in Vitro[J].Nongchanpin Jlagong.Xuekan,2010(9). |
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| Authors: | Luo Shishu Zhang Haide Liu Xiaoling Zhu Li |
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| Institution: | Luo Shishu,Zhang Haide,Liu Xiaoling,Zhu Li(College of Food Science,Hainan University,Haikou 570228,China) |
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| Abstract: | This article used ultrasonic extraction,ethanol extraction,supercritical CO2 extraction to extract arecoline from areca nut.The antimicrobial effect of arecoline extracted by three methods was studied in vitro against Escherichia coli,Staphylococ-cus aureus,Bacillus Proteus,Bacillus sublilis,Candida albicans,Bacillus anthracis,Penicillium.The experimental results showed:the extractive from areca nut showed the good effect for Bacillus Proteus,Candida albicans,Bacillus anthracis,the minimum ultrasonic extrac... |
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| Keywords: | ultrasonic extraction ethanol extraction supercritical CO2 extraction arecoline antimicrobial activity |
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