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三种草花种子寄藏真菌检测与鉴定
引用本文:吴利敏,赵梁军,李健强,刘西莉,刘耀忠.三种草花种子寄藏真菌检测与鉴定[J].种子,2004,23(7):3-6,9.
作者姓名:吴利敏  赵梁军  李健强  刘西莉  刘耀忠
作者单位:1. 中国农业大学观赏园艺与园林系,北京,100094
2. 中国农业大学植物病理学系,北京,100094
3. 北京市园林科学研究所,北京,100102
基金项目:北京市科技新星计划项目
摘    要:采用平皿培养方法分别对三种草花共计9个品种的种子样品进行了种子寄藏真菌检测和纯化、鉴定.结果表明,一串红两个品种的种子样品寄藏真菌带菌率最高,分别达到72.5%和54.0%,万寿菊三个品种的种子样品寄藏真菌带菌率分别为11.5%、13.0%和11.5%,矮牵牛四个品种的种子样品带菌率在17.0%~50.5%之间变化.三种草花种子寄藏真菌共有9种类型,根据其形态特征鉴定为链格孢菌(Alternaria alternata、A.tenuissina、A.tenuissima)、毛壳菌(Chaetomium sp.)、青霉属(Penicillium sp.),编号为B、C、G、H的四种分离物的分类地位待定.研究结果表明,链格孢菌是一串红、万寿菊和矮牵牛三种草花种子寄藏真菌的优势菌群.

关 键 词:草花  种子  寄藏真菌  健康检测  真菌鉴定

Testing and Identifying of Seed-Borne Fungi in Three Annual Flowers
Wu limin,Zhao liangjun,Li jianqiang,Liu xili,Liu yaozhong.Testing and Identifying of Seed-Borne Fungi in Three Annual Flowers[J].Seed,2004,23(7):3-6,9.
Authors:Wu limin  Zhao liangjun  Li jianqiang  Liu xili  Liu yaozhong
Institution:Wu limin 1 Zhao liangjun 1 Li jianqiang 2 Liu xili 2 Liu yaozhong 3
Abstract:Seed -borne fungi of nine cultivars collected from threeannual flowers were determined by means of perti-dish testing.method The isolations were purified and identified. The results showed that the pathogens were detected in all the cultivars. The rate of seed-borne fungi inSalvia splendenswas the highest and the lowest in Tagetes erecta. The percentage of seed-borne fungi varied from 11.3%to 72.5%. Accroding to the morphology, the isolations were confirmed as Alternaria(including A.alternata?A.tenuissina?A.tenuissima), which was the dominant species in the experimental cultivars, Chaetomium, Penicillium and other four species(B?C?G?H) which had not been identified.for their classification.
Keywords:Annual flowers  Seeds  Health testing  Identifying  Eeed-borne fungi
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