| 西洋杜鹃叶片离体培养及植株再生 |
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| 引用本文： | 刘燕,陈训.西洋杜鹃叶片离体培养及植株再生[J].种子,2008,27(11). |
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| 作者姓名： | 刘燕 陈训 |
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| 作者单位： | 1. 贵州省生物研究所,贵阳,550006
2. 贵州科学院,贵阳,550001 |
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| 基金项目： | 国家农业成果转化基金，贵州省科技攻关项目 |
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| 摘 要： | 以西洋杜鹃的嫩叶片为外植体，研究了其离体培养和植株再生的过程。结果表明：叶片愈伤组织诱导最佳培养基为Read＋ZT5．0mg／L，可用回归模型y=19．21＋7．32x1来估测西洋杜鹃叶片愈伤组织诱导情况。叶片愈伤组织增殖培养基同初代培养基，继代30d后体积可增为原来的3倍，重量增为原来的2．5～4．5倍。愈伤组织分化的最佳培养基配方为：Read＋ZT1．0mg／L＋NAA0．01mg／L，分化率为87．5％，分化出苗数在12—18株。生根培养基以Read＋IBA1．0mg／L＋NAA2．0mg／L＋ZT 0．2mg／L为好，生根率迭90％以上。炼苗基质选腐殖土：黄沙：珍珠岩：5：1：1为宜，存活率达88．33％。
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| 关 键 词： | 西洋杜鹃 叶片 离体培养 植株再生 |
Induction of Callus from Leaf Explants of Rhododendron hybridum and Its Regeneration |
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| LIU Yan,CHEN Xun.Induction of Callus from Leaf Explants of Rhododendron hybridum and Its Regeneration[J].Seed,2008,27(11). |
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| Authors: | LIU Yan CHEN Xun |
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| Abstract: | The technique system of induction of callus from leaf explants of Rhododendron hybridum and its re- generation were established.The results showed that Read+ZT 5.0 mg/L was the best medium to induce cal- lus from leaf explants.And Linear regression model y=19.21+7.32x_1 could estimate the growth condition of callus.Multiplication medium was the same as induction medium of callus.After 30 d inoculated,callus reached 3 times of its volume,2.5-4.5 times of its weight.Read+ZT 1.0 mg/L+NAA 0.10 mg/L was the best medium inducing callus to differentiate shoots and a high frequency could reach 87.5 %.The regenerated shoots rooted readily on Read medium plus 1.0 mg/L IBA 2.0 mg/L NAA and ZT 0.2 mg/L.The rate of roo- fing could reach 90%.The regenerate plants were acclimatized the soil of muck,yellow sand and perlite (5:1:1),the survival rate of plandets was 88.33%. |
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| Keywords: | Rhododendron hybridum leaf inoculating in vitro plant regeneration |
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