| 类猪圆环病毒因子P1核酸链型和极性的研究 |
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| 引用本文： | 温立斌,何孔旺,倪艳秀,张雪寒,李彬,王小敏,郭容利,周俊明,俞正玉,茅爱华,吕立新.类猪圆环病毒因子P1核酸链型和极性的研究[J].华北农学报,2012(3):120-122. |
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| 作者姓名： | 温立斌 何孔旺 倪艳秀 张雪寒 李彬 王小敏 郭容利 周俊明 俞正玉 茅爱华 吕立新 |
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| 作者单位： | 江苏省农业科学院兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心 |
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| 基金项目： | 国家自然科学基金(30972184);江苏省自然科学基金(BK2008351) |
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| 摘 要： | 研究报道类猪圆环病毒因子P1的核酸链型和极性的研究结果。采用氯化铯平衡密度梯度离心获得较为纯净的病毒粒子后,提取病毒DNA,经S1核酸酶消化、特异性单引物第一轮PCR扩增结合常规第二轮PCR扩增等研究,结果表明,类猪圆环病毒因子P1为单股、负链基因组。
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| 关 键 词： | 类猪圆环病毒因子P1 核酸链型 极性 |
Studies on the Nucleic Acid Strandedness and Polarity of Porcine Circovirus-like Agent P1 |
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| Institution: | WEN Li-bin,HE Kong-wang,NI Yan-xiu,ZHANG Xue-han,LI Bin,WANG Xiao-min, GUO Rong-li,ZHOU Jun-ming,YU Zheng-yu,MAO Ai-hua,LU Li-xin(Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture,National Center for Engineering Research of Veterinary Bio-products,Nanjing 210014,China) |
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| Abstract: | The results of the nucleic acid strandedness and polarity of porcine circovirus-like agent P1 were reported here.Relatively pure viral particles were obtained by CsCl equilibrium density-gradient centrifugation.The P1 could be determined as single strand nucleic acid and negative-strand genome by S1 nuclease treatment and two round of PCR amplification,the first round using the single-stranded-specific primer and the second round by one pair of specific primers. |
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| Keywords: | Porcine circovirus-like agent P1 Nucleic acid strandedness Polarity |
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