| 导入AGPase基因的转基因可育水稻及其经济性状的研究 |
| |
| 引用本文： | 宋敏,李援亚,张云孙.导入AGPase基因的转基因可育水稻及其经济性状的研究[J].华北农学报,2001,16(4):11-14. |
| |
| 作者姓名： | 宋敏 李援亚 张云孙 |
| |
| 作者单位： | 云南大学 生物技术系,云南,昆明,650091 |
| |
| 基金项目： | 云南省自然科学基金重点项目 (96C0 0 3Z) |
| |
| 摘 要： | 以农杆菌介导法将淀粉合成关键酶AGPase(腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，41，42中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。
|
| 关 键 词： | AGPase基因 根癌农杆菌 水稻 淀粉含量 千粒重 转基因 品种改良 |
| 文章编号： | 1000-7091(2001)04-0011-04 |
| 修稿时间： | 2000年10月17 |
Studies on Transgenic Rice(Oryza sativa L.)Plants Transformed with AGPase Gene and Its Economic Characters |
| |
| SONG Min,LI Yuan ya,ZHANG Yun sun.Studies on Transgenic Rice(Oryza sativa L.)Plants Transformed with AGPase Gene and Its Economic Characters[J].Acta Agriculturae Boreali-Sinica,2001,16(4):11-14. |
| |
| Authors: | SONG Min LI Yuan ya ZHANG Yun sun |
| |
| Abstract: | AGPase gene,the critical starch synthesis enzyme gene,was transformed into high class rice No.35,38,39,41,42 by Agrobacterium mediated transformation and fertile transgenic rice plants were obtained.The results of PCR indicated that the foreign gene had been integrated into the rice genome.The research showed that 1000 grain weight of transgenic plants was obviously improved,but the starch content of the rice was little changed. |
| |
| Keywords: | AGPase gene Agrobacterium tumefaciens Polymerase chain reaction(PCR) The starch content of rice 1000 grain weight |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
