| PEDV亚单位疫苗免疫增强剂的筛选 |
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| 引用本文： | 陈瑾,黄春娟,于晓明,侯立婷,乔绪稳,郑其升,侯继波.PEDV亚单位疫苗免疫增强剂的筛选[J].华北农学报,2016(2):205-210. |
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| 作者姓名： | 陈瑾 黄春娟 于晓明 侯立婷 乔绪稳 郑其升 侯继波 |
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| 作者单位： | 江苏省农业科学院，国家兽用生物制品工程技术研究中心，江苏 南京 210014; 江苏省动物重要疫病与人兽共患病防控协同创新中心，江苏 扬州 225009 |
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| 基金项目： | 江苏省农业科技自主创新资金项目(CX(15)1062)；公益性行业(农业)科研专项(201303046) |
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| 摘 要： | 为了研究PEDV的新型亚单位疫苗,提高PEDV亚单位疫苗的免疫原性,选取免疫增强剂CVC1302、CVC1303与国家兽用生物制品工程技术研究中心构建的可溶性表达PEDV S蛋白核心表位COE的重组蛋白(p QZCOE-LTB)混合乳化制成疫苗,免疫4周龄的ICR小鼠,首免14 d后加免1次,随后采集首免14(加强免疫前),21,28,55 d的小鼠血清,分别用琼扩试验的方法定性检测血清IgG抗体以及用ELISA试剂盒定量检测小鼠血清IgG抗体滴度动态变化,肠组织sIgA抗体滴度。结果表明,重组蛋白与CVC1303配合使用免疫小鼠能产生显著高于灭活苗(CV777)阳性对照组的IgG和sIgA抗体水平,说明CVC1303免疫增强剂能有效增强该重组蛋白的免疫原性,提高亚单位疫苗免疫效果。
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| 关 键 词： | 猪流行性腹泻病毒 核心抗原表位 免疫增强剂 免疫效力 |
Screening the Immune Enhancer of Porcine epidemic diarrhea virus Subunit Vaccine |
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| Abstract: | This study successfully constructed pQZ-COE-LTB expression vector and expressed the soluble re-combinant protein,which was chosen for PEDV subunit vaccine candidate.In order to enhance the immunogenicity of the PEDV subunit vaccine,we selected CVC1302 and CVC1303 as immune enhancer,combined with PEDV sub-unit vaccine.Immunize 4-week-old ICR mice,second immunize conducted after 14 d,then collected the 14,21 ,28, 55 d mouse serum after first immune.Agar diffusion test and ELISA kits of detection of serum IgG antibody levels, intestinal tissue sIgA levels were conducted.The results showed that PEDV subunit vaccine combined with CVC1303 immune enhancer group produced significantly higher levels of IgG and sIgA antibodies than CV777 inac-tivated vaccine positive control group,indicating CVC1303 immune enhancer can effectively enhance the immunoge-nicity of the PEDV subunit vaccine. |
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| Keywords: | PEDV COE Immune enhancer Immunogenicity |
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