| HPLC法测定南五味子中五味子醇甲和五味子酯甲的含量 |
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| 引用本文： | 谭晓虹,田嘉铭,王治宝,杨晓媛,马宁.HPLC法测定南五味子中五味子醇甲和五味子酯甲的含量[J].河北北方学院学报(自然科学版),2011,27(6):70-72,75. |
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| 作者姓名： | 谭晓虹 田嘉铭 王治宝 杨晓媛 马宁 |
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| 作者单位： | 1. 河北北方学院基础医学院药学系,河北张家口,075000
2. 河北北方学院药学系2006级,河北张家口,075000 |
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| 摘 要： | 目的建立快速、准确的测定南五味子有效成分五味子醇甲和五味子酯甲含量的方法．方法五味子醇甲和五味子酯甲的含量测定采用高效液相色谱法（HPLC），色谱条件：Hypersil BDSC8柱（4．6mm×150mm，5μm），检测波长254nm，柱温30℃，流速1．0mL·min-1，流动相：甲醇一0．1％醋酸（65：35），...
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| 关 键 词： | 南五味子 五味子醇甲 五味子酯甲 HPLC |
Determination of Schisandrin and Schisantherin A in Schisandra sphenanthera Rehd. et Wils. by HPLC |
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| Institution: | TAN Xiao-hong1,TIAN Jia-ming1,WANG Zhi-bao1,et al(Department of Pharmacy,Hebei North University,Zhangjiakou 075000,Hebei,China) |
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| Abstract: | Objective To investigate a rapid and sensitive method for determination of schisandrin and schisantherin A in Schisandra sphenanthera Rehd. et Wils. Methods To analyse Schisandrin and Schisantherin A in Schisandra sphenanthera Rehd. et Wils. by high performance liquid chromatography （HPLC） on a Hypersil BDSC8 column （4. 6 min×150 mm, 5 μm）, detection wavelength was set at 254 nm and the column temperature was 30 ℃, The mobile phase was methanoi-0.1% acetic acid （65： 35）, with the flow rate of 1.0mL · min-1. Results Schisandrin showed a good linear relationship at range of 4. 950-15.43ng ·μL-1 （r=0. 9996）, and Schisantherin A showed a good linear relationship at range of 11.38;174. 6 ng ·μL-1 （r= 0. 9999）. The average recovery rate schisandrin and schisantherin A in Schisandra sphenanthera Rehd. et Wils. were 98. 79% and 99.23;, and RSD of the method were 1.82% and 2.20; respectively; Conclusion The method is proved to be accurate, rapid, and sensitive. It can be used for quality control of schisandrin and schisantherin A in Schisandra sphenanthera Rehd. et Wils. |
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| Keywords: | Schisandra sphenanthera Rehd et Wils schisandrin schisantherin A HPLC |
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