| 冷休克诱导黄河鲇(Silurus asotus)二倍体雌核发育(英文) |
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| 作者单位： | CHENG Guo，LI Xue-jun，QIAO Zhi-gang，SHI Ling，PENG Xin-liangCollege of Life Science，Henan Normal University，Xinxiang 453007 |
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| 摘 要： | Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. Mthod] The Silurus astus sperms were irradiated by ultraviolet,then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus.Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization,and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5%). Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files for further study of gynogenesis development mechanism.
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Induction of Gynogenesis of Silurus astus Using Cold Shock |
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| Authors: | CHENG Guo LI Xue-jun QIAO Zhi-gang SHI Ling PENG Xin-liangCollege of Life Science Henan Normal University Xinxiang |
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| Institution: | CHENG Guo,LI Xue-jun,QIAO Zhi-gang,SHI Ling,PENG Xin-liangCollege of Life Science,Henan Normal University,Xinxiang 453007 |
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| Abstract: | Objective] The cold shock method was used to induce diploid gynogenesis of Silurus astus. Mthod] The Silurus astus sperms were irradiated by ultraviolet,then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus.Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization,and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5%). Conclusion] The experiment laid foundation for culturing new excellent Silurus astus variety and accumulated original files for further study of gynogenesis development mechanism. |
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| Keywords: | Silurus asotus Gynogenesis Diploid Cold shock |
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