| Study on DNA Cytosine Methylation of Cotton （Gossypium hirsutum L.） Genome and Its Implication for Salt Tolerance |
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| 作者姓名： | ZHAO Yun-lei YU Shu-xun YE Wu-wei WANG Hong-mei WANG Jun-juan FANG Bao-xing |
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| 作者单位： | Cotton Research Institute, Chinese Academy of Agricultural Sciences （CAAS）/Key Laboratory of Cotton Genetic Improvement, Ministry ofAgriculture, Anyang 455000, P.R. China |
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| 基金项目： | Acknowledgements This work was supported by the Basic R＆D Operation Special Fund for the Central Level, Non-Profit, Scien- tific Research Institutes, China （SJB0802）. |
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| 摘 要： | To study the relations between DNA methylation and abiotic stress responses in cotton （Gossypium hirsutum L.）,the methylation-sensitive amplified polymorphism （MSAP） method was used to investigate the differences in methylation level and the change of cytosine methylation patterns under salt （NaCl） stress in two different salt-tolerant cotton lines.The results showed that the number of the cytosine methylation of CCGG sites in high salt-tolerant cotton line was less than that in low salt-tolerant line.Under salt stress,extensive cytosine methylation alterations including hypermethylation and demethylation as well as the potential conversion of methylation types occurred in the salt-treated cotton line compared with the corresponding control.Interestingly,salt stress-induced demethylation loci that occurred in high salt- tolerant cotton line were greater than those in low salt-tolerant cotton line,however,salt stress-induced hypermethylation loci in the high salt-tolerant cotton line were less than those in low salttolerant cotton line.It suggested that the demethylation positively contributed to salt tolerance and the hypermethylation had negative effect on salt tolerance in cotton.
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| 关 键 词： | DNA甲基化 耐盐性 胞嘧啶 陆地棉 棉花 基因组 扩增多态性 甲基化水平 |
| 收稿时间： | 14 July 2009 |
Study on DNA Cytosine Methylation of Cotton(Gossypium hirsutum L.) Genome and Its Implication for Salt Tolerance |
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| ZHAO,Yun-lei YU,Shu-xun YE,Wu-wei WANG,Hong-mei WANG,Jun-juan FANG,Bao-xing.Study on DNA Cytosine Methylation of Cotton(Gossypium hirsutum L.) Genome and Its Implication for Salt Tolerance[J].Agricultural Sciences in China,2010,9(6):783-791. |
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| Authors: | ZHAO Yun-lei YU Shu-xun YE Wu-wei WANG Hong-mei WANG Jun-juan FANG Bao-xing |
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| Institution: | 1. INRA, UMR 1095 GDEC-UBP, 5 Chemin de Beaulieu, F-63039 Clermont-Ferrand, France;2. COMSATS Institute of Information Technology, Department of Biosciences, Park Road, 45550 Islamabad, Pakistan;3. INRA, UR1268 BIA Biopolymères, Interactions Assemblages, F-44316 Nantes, France;4. INRA, QPA Plateforme Protéomique, F-63122 Saint-Genès Champanelle, France |
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| Abstract: | To study the relations between DNA methylation and abiotic stress responses in cotton(Gossypium hirsutum L.),the methylation-sensitive amplified polymorphism(MSAP)method was used to investigate the differences in methylation level and the change of cytosine methylation patterns under salt(NaCl)stress in two different salt-tolerant cotton lines.The results showed that the number of the cytosine methylation of CCGG sites in high salt-tolerant cotton line was less than that in low salt-tolerant line.Under salt stress,extensive cytosine methylation alterations including hypermethylation and demethylation as well as the potential conversion of methylation types occurred in the salt-treated cotton line compared with the corresponding control.Interestingly,salt stress-induced demethylation loci that occurred in high salt-tolerant cotton line were greater than those in low salt-tolerant cotton line,however,salt stress-induced hypermethylation loci in the high salt-tolerant cotton line were less than those in low salt-tolerant cotton line.It suggested that the demethylation positively contributed to salt tolerance and the hypermethylation had negative effect on salt tolerance in cotton. |
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| Keywords: | cotton DNA methylation salt stress MSAP methylation polymorphism |
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