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鸡新城疫病毒地方野毒株的分离鉴定与致病性试验
引用本文:吴志明,王婷,程相朝,吴庭才,李清利,马淑蕾.鸡新城疫病毒地方野毒株的分离鉴定与致病性试验[J].河南科技大学学报(农学版),2004,24(4):24-25,39.
作者姓名:吴志明  王婷  程相朝  吴庭才  李清利  马淑蕾
作者单位:1. 河南省兽医防治站,河南,郑州450002
2. 河南科技大学,动物科技学院,河南,洛阳,471003
3. 汝州市动检站,河南,汝州,467500
基金项目:河南省高校青年骨干教师资助项目(2001193)
摘    要:从某一疑似新城疫病例的蛋鸡群中分离到一株病毒,经HA和HI试验、血清中和试验和动物回归试验。最终确认所分离毒株为新城疫病毒。经对分离株的MDT、ICPI和IVPI等致病指数的测定,表明该毒株为新城疫强毒株。

关 键 词:  新城疫病毒  分离  鉴定  致病性  地方野毒株  新城疫
文章编号:1008-4673(2004)04-0024-02
修稿时间:2004年7月12日

Isolation and Identification of the chicken newcastle disease virus
WU Zhi-ming,WANG Ting,CHENG Xiang-chao,WU Ting-cai,LI Qing-li,MA Shu-lei.Isolation and Identification of the chicken newcastle disease virus[J].Journal of Luoyang Agricultural College,2004,24(4):24-25,39.
Authors:WU Zhi-ming  WANG Ting  CHENG Xiang-chao  WU Ting-cai  LI Qing-li  MA Shu-lei
Institution:WU Zhi-ming~1,WANG Ting~2,CHENG Xiang-chao~2,WU Ting-cai~2,LI Qing-li~3,MA Shu-lei~2
Abstract:A local virus strain is isolated from some chicken which are suspected being infected with NDV.It is identified as NDV by hemagglutination test,hemagglutination inhibition test,and inoculation of embryos after neutralization using particular sera and challenge with 2-week chicken.Determination on mean death time (MDT) of embrous,intracerebral pathogenicith index(ICPI) and intrveinal pathogenicity index(IVPI) of the isolation is corried out .The results show that the isolated NDV strain is highly virulent pathogenicity.
Keywords:Chicken  NDV  Isolation and identification  Pathogenic
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