| LAMP、荧光PCR和标准PCR对6种水产疫病的检测能力比较 |
| |
| 引用本文： | 赵丽娟,陈鹏,黎烽,车行,关海涛,唐姝,林蠡.LAMP、荧光PCR和标准PCR对6种水产疫病的检测能力比较[J].仲恺农业工程学院学报,2022,35(3):37-43. |
| |
| 作者姓名： | 赵丽娟 陈鹏 黎烽 车行 关海涛 唐姝 林蠡 |
| |
| 摘 要： | 随着全球水产养殖产业的蓬勃发展，水产动物疫病频繁发生，给水产产业带来巨大经济损失. 快速检测技术在防控水产疫病中具有重要的意义. 基于 LMAP 法与荧光定量 PCR 法为原理设计的水产病害快速检测试剂盒，凭借其快速高效的检测能力在基层检测中得到了广泛应用，为评价这些新兴快检产品的检出能力和判定准确性，本试验分别采用 LAMP、荧光 PCR 和标准 PCR 对6种水产疫病的进行比较检测. 结果表明，3种检测方法的检测能力排序是：荧光PCR快速检测>标准PCR>LAMP，但是三者判定准确度一致，说明3种检测方法在检测水产动物疾病中是可靠的.
|
| 收稿时间： | 2022-09-30 |
Detectability comparisons of LAMP,fluorescent PCR and standard PCR methods for six aquatic diseases |
| |
| Abstract: | With the rapid development of global aquaculture industry, aquatic animal diseases have occurred frequently, which cause huge economic losses to the aquaculture industry. Rapid detection method plays a critical role in the prevention and control of aquatic animal diseases. Due to their high speed and efficient detection, the rapid detection kits designed based on the principle of LMAP method and fluorescent PCR method have been widely used in the detection of aquatic animal diseases. In order to investigate the detection capability and accuracy of these rapid detection methods, six kinds of aquatic animal diseases were subjected to the detection using LMAP, fluorescent PCR and standard PCR methods in this study. The results showed that the order of detectability was fluorescent PCR>standard PCR>LAMP. However, the judging accuracy of these three methods is consistent, indicating that these three methods are all reliable when they are applied in detection of aquatic animal diseases. |
| |
| Keywords: | |
|
| 点击此处可从《仲恺农业工程学院学报》浏览原始摘要信息 |
| 点击此处可从《仲恺农业工程学院学报》下载免费的PDF全文 |
|
