| 基于COⅠ基因序列的裸颊鲷属鱼类进化关系探讨 |
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| 引用本文： | 张癸新,叶嘉文,陈厚桦,李江涛,李清清,梁日深,林蠡.基于COⅠ基因序列的裸颊鲷属鱼类进化关系探讨[J].仲恺农业工程学院学报,2021,34(3):48-54. |
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| 作者姓名： | 张癸新 叶嘉文 陈厚桦 李江涛 李清清 梁日深 林蠡 |
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| 摘 要： | 为从分子水平分析我国裸颊鲷属(Lethrinus)鱼类系统分类关系，同时探讨线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因作为分子标记在裸颊鲷属鱼类物种鉴定和亲缘关系的可信性.本研究通过PCR扩增及测序获得了我国南海10种裸颊鲷COⅠ基因部分序列，结合GenBank下载的2种裸颊鲷最后共12种37个个体线粒体COⅠ基因序列，利用MEGA5.0计算序列的系统进化位点，基于最大似然法构建分子系统进化树．结果表明：12种裸颊鲷种内遗传距离为0.000~0.005，平均遗传距离为0.002，远低于物种鉴定最小种间遗传距离0.020(2%)；种间遗传距离为0.074~0.210，平均遗传距离为0.155，种间遗传距离是种内遗传距离77.5倍，表明COⅠ基因可作为裸颊鲷鱼类物种鉴定条形码基因．构建的进化树上，12种裸颊鲷属鱼类主要形成4个分支，长吻裸颊鲷最先分化，单独形成一支(分支Ⅳ)，位于进化树基部. 其次是红鳍裸颊鲷也单独形成一支(分支Ⅲ). 剩下的裸颊鲷主要聚为两支：分支I包含5种裸颊鲷，该分支裸颊鲷大部分体型较低，牙齿为犬牙状齿；分支II包含5种裸颊鲷，该分支裸颊鲷体型较高，牙齿为臼状齿，与前人研究将裸颊鲷基本分为高体型臼状齿，低体型犬牙状齿两个类群结果一致.也支持前人推断认为裸颊鲷祖先先形成高体型犬牙状齿特征，后期进化过程中逐渐分化出高体型臼状齿及低体型圆锥状齿的类群的观点.
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| 收稿时间： | 2021-11-02 |
Discussion on the evolutionary relationship of the fishes of the genus
Lethrinus based on the COⅠ gene sequence |
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| Abstract: | To investigate the availability of using COⅠ sequences as DNA barcodes to identify Lethrinus species, partial sequences of mitochondrial cytochrome oxidase subunit Ⅰ (CO Ⅰ) gene of 10 Lethrinus speices from South China Sea were obtained by PCR amplification and sequencing. Combining with COⅠ gene sequence of 2 Lethrinus species from GenBank, a total of 37 individuals COⅠ sequences of 12 Lethrinus species were obtained. The phylogenetic of the sequence was calculated using MEGA5.0, and the molecular phylogenetic relationships were constructed and analyzed using maximum likelihood method.The results showed that intra-specific genetic distance within the 12 Lethrinus species were between 0.000 to 0.005 with the average genetic distance was 0.002, lower than the minimum inter-specific genetic distance of 0.020(2%) for species identification. The inter-species genetic distances among species were 0.074-0.210, with the average genetic distance was 0.155, which was 77.5 times of intra-specific genetic distance, Indicating that the COⅠ gene could be used as the barcode gene for the Lethrinus identification. In the molecular phylogenetic tree, 12 Lethrinus species mainly formed 4 groups. Specie Lethrinus miniatus was first seperated and located at the base of the tree (branch Ⅳ). Following was Lethrinus miniatus which also fromed a single branch (branch Ⅲ). The rest of Lethrinus species were divided into two branches: Branch Ⅰ contained 5 Lethrinus species, most of which were low-bodied and conical teeth; Branch Ⅱ contained 5 Lethrinus species with higher bodies and molariform and submolariform teeth. Results were consistent with the previous studies that Lethrinus species were mainly divided into two groups: one was high bodied with mortar teeth feature and another was low-bodied with conical teeth. It also supported the previous conclusion that the two different types of Lethrinus(low-ied with conical teeth and low-bodied with conical teeth)both evolved from a high-bodied, conical-toothed ancestor like species Lethrinus miniatus. |
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