| 豹纹鳃棘鲈哈维氏弧菌的分离鉴定与耐药分析 |
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| 引用本文： | 孔明慧,黄丽芳,廖国威,林灿佳,符精仁,李子博,梁日深,林蠡.豹纹鳃棘鲈哈维氏弧菌的分离鉴定与耐药分析[J].仲恺农业工程学院学报,2021,34(4):16-20. |
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| 作者姓名： | 孔明慧 黄丽芳 廖国威 林灿佳 符精仁 李子博 梁日深 林蠡 |
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| 摘 要： | 为检测与鉴定感染豹纹鳃棘鲈（Plectropomus leopardus）体表溃烂病的病原菌种类，本研究从患病的豹纹鳃棘鲈中分离疑似病菌，通过形态学观察、生理生化检测并结合16S rRNA序列分析进行鉴定. 结果表明，分离的菌株为哈维氏弧菌（Vibrio harveyi）. 通过纸片琼脂扩散法对18种抗生素进行药物敏感试验，发现病原菌对氟苯尼考最敏感，对头孢唑啉、新霉素等6种药物中度敏感，对羧苄西林、头孢氨苄等11种药物具有抗性. 通过二倍稀释法对4种常用抗生素的最小抑菌浓度进行测定，结果表明氟苯尼考抑菌效果最强，最小抑菌浓度为0.5200 μg/mL.
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Isolation,identification and drug resistance analysis of
Vibrio harveyi from Plectropomus leopardus |
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| Abstract: | In order to detect and identify the pathogen of the skin ulceration disease of Plectropomusleopardus, the bacteria were isolated from diseased fish and identified as Vibrio harveyi basing on morphological characteristics, physiological and biochemical identification as well as 16S rRNA gene sequence analysis. The drug sensitive tests of 18 kinds of antibiotics were carried out using Kirby-bauer way. Results showed that the bacteria were most sensitive to Florfenicol, intermediate sensitive to 6 antibiotics including Cefazolin and Neomycin, and resistant to 11 antibiotics including Carbenicillin and Cefalexin. The minimum inhibitory concentration (MIC) of 4 selected antibiotics were also determined using double dilution method. The result revealed that the Florfenicol showed the best inhibiting effect among the 4 antibiotics and the MIC was 0.5200 μg/mL. |
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