| 海南野生三斑石斑鱼染色体核型分析 |
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| 引用本文： | 蔡岩,周永灿,张本,谢珍玉,王世锋.海南野生三斑石斑鱼染色体核型分析[J].热带生物学报,2011(2):97-100. |
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| 作者姓名： | 蔡岩 周永灿 张本 谢珍玉 王世锋 |
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| 作者单位： | [1]海南大学热带生物资源教育部重点实验室,海南海口570228 [2]海南大学海洋生物实验教学示范中心,海南海口570228 |
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| 基金项目： | 国家自然科学基金项目（31060355）; 农业科技成果转化项目（2009GB2E200302）; 海洋渔业科学与技术浙江省重中之重学科开放课题资助项目（20100115）; 海南大学科研启动基金资助项目（kyqd1017）;海南大学“211工程”三期建设项目 |
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| 摘 要： | 以头肾组织为材料,采用植物血球凝集素（phytohemagglutinin,PHA）、秋水仙素溶液腹腔注射和空气干燥制片法,对海南野生三斑石斑鱼（Epinephelus trimaculatus）的染色体组型进行研究,结果表明,海南野生三斑石斑鱼具有48条染色体,其核型公式为2n=48,48t,臂数NF=48。未发现与性别相关的异型染色体。基于目前已研究过的23种石斑鱼核型进行统计,推测2n=48,48t,NF=48可能为石斑鱼属的基本原始核型。
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| 关 键 词： | 三斑石斑鱼 染色体 核型 |
Chromosome Karyotype Analysis of E.trimaculatus（Valenciennes,1828） |
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| CAI Yan,ZHOU Yong-can,ZHANG Ben,XIE Zhen-yu,WANG Shi-feng.Chromosome Karyotype Analysis of E.trimaculatus（Valenciennes,1828）[J].Journal of Tropical Biology,2011(2):97-100. |
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| Authors: | CAI Yan ZHOU Yong-can ZHANG Ben XIE Zhen-yu WANG Shi-feng |
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| Institution: | 1.Key Laboratory of Tropical Biological Resources of Ministry of Education,Hainan University,Haikou 570228,China; 2.Experimental Teaching Demonstration Center of Marine Biology,Hainan University,Haikou 570228,China) |
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| Abstract: | In our study,the head kidney tissue were used as materials,and in vivo treatment by phytohemagglutinin（PHA） and colchicines and Giemsa staining were performed,and the cytogenetics of E.trimaculatus were analyzed.The results indicated that the karyotypic formula of E.trimaculatus was 2n=48,48t,NF=48,no heteromorphic chromosome was detected.Based on the karyotypes of 23 species of groupers,the conclusion that 2n=48,48t,NF=48 was the primitive cytogenetic characterization of genus Epinephelus were proposed. |
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| Keywords: | Epinephelus trimaculatus chromosome karyotype |
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