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大羽藓形态学性状及挥发性成分的GC-MS分析
引用本文:秦鑫婷,杨琳,许琼玉,胡旭,王新广,张莉娜.大羽藓形态学性状及挥发性成分的GC-MS分析[J].热带生物学报,2020,11(2):163.
作者姓名:秦鑫婷  杨琳  许琼玉  胡旭  王新广  张莉娜
作者单位:1.海南大学 热带生物资源教育部重点实验室,海口 570228
基金项目:海南省自然科学基金项目(318MS018);国家自然科学基金项目(31760054);海南省高等学校科学研究项目(Hnky2017ZD-6);海南大学热带农林学院生物学学科优秀研究生培养项目
摘    要:为探究传统中药大羽藓的形态学特征及挥发性化学成分,以海南黎母山的大羽藓(Thuidium cymbifolium (Dozy et Molk.) Dozy et Molk.)为研究材料,对其形态性状进行了描述,比较了该种与4个近缘种或变种的形态差异,并采用石油醚快速溶剂萃取技术结合GC-MS法对其挥发性物质进行萃取和化学成分分析。共鉴定出31种挥发性成分,占总组分的91.1%,主要成分类型包括酯类(25.48%),萜类(22.64%),酮类(20.64%)及甾醇类(10.61%);主要成分为丁酸丁酯(18.96%)、4?庚酮(10.90%)、角鲨烯(10.75%)和3?甲基?4?庚酮(9.74%)。

关 键 词:大羽藓    形态学性状    挥发性成分    气相色谱?质谱
收稿时间:2019-11-05

Morphology of Thuidium cymbifolium and GC-MS Analysis of Its Volatile Compounds
Institution:1.Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, Hainan 5702282.Center for Terrestrial Biodiversity of the South China Sea, Hainan University, Haikou, Hainan 5702283.College of Life and Pharmaceutical Sciences, Hainan University, Haikou, Hainan 5702284.College of Tropical Crops, Hainan University, Haikou, Hainan 5702285.College of Horticulture, Hainan University, Haikou, Hainan 570228, China
Abstract:Thuidium cymbifolium, a traditional medicine, was collected from Limu Mountain in Hainan to observe its morphology and analyze its volatile composition. Its morphology was compared with that of its 4 relatives and varieties to find out their morphological difference, and its volatile compounds was extracted by using accelerated solvent extraction (ASE) with petroleum ether as solvent and then analyzed by using gas chromatography-mass spectrometry (GC-MS). The analysis showed that the volatile compounds of T. cymbifolium were identified to contain 31 constituents, accounting for 91.1% of the total chemical composition. The main constituents were esters (25.48%), terpenes (22.64%), ketones (20.64%) and sterols (10.61%), including butyl butyrate (18.96%), 4-heptanone (10.90%), squalene (10.75%) and 4-heptanone,3-methyl (9.74%).
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