| 猪胸膜肺炎放线杆菌基因组文库的构建 |
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| 引用本文： | 杨建德,刘燕霏,徐军.猪胸膜肺炎放线杆菌基因组文库的构建[J].天津农学院学报,2008,15(3):1-3. |
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| 作者姓名： | 杨建德 刘燕霏 徐军 |
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| 作者单位： | 天津农学院,动物科学系,天津,300384 |
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| 基金项目： | 天津市自然科学基金，国家留学回国人员科研启动基金 |
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| 摘 要： | 由猪胸膜肺炎放线杆菌（APP）引起的猪传染性胸膜肺炎是猪最重要的呼吸道传染病之一。为研究该细菌表面蛋白的结构与功能，选取APP代表菌株，提取其基因组DNA，用TSPS091随机消化成3～8kb的片段并回收，连接到预先消化的入ZAPⅡ载体上，经过包装、扩增和滴定后，成功地构建了APP基因组文库。
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| 关 键 词： | 猪胸膜肺炎放线杆菌 基因组文库 构建 |
Construction of genome library of Actinobacillus pleuropneumoniae |
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| YANG Jian-de,LIU Yan-fei,XU Jun.Construction of genome library of Actinobacillus pleuropneumoniae[J].Journal of Tianjin Agricultural College,2008,15(3):1-3. |
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| Authors: | YANG Jian-de LIU Yan-fei XU Jun |
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| Institution: | (Department of Animal Science, Tianjin Agricultural University, Tianjian 300384, China) |
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| Abstract: | Porcine contagious pleuropneumoniae, which was one of the most important infectious respiratory diseases, was caused by actinobacillus pleuropneumoniae. In order to study the structures and functions of APP surface proteins, the genome library was constructed. The genome was extracted from the representative APP strain, and randomly digested by TSP5091 into 3～8 kb fragments. The genome library was successfully constructed by ligation of recovered fragments to predigested lambda ZAPII after packaging, amplification and titration. |
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| Keywords: | Actinobacilluspleuropneumoniae genomelibrary construction |
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