| 耐药型大肠杆菌引发的仔猪大肠杆菌病的PCR快速鉴定 |
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| 引用本文： | 王选,赵瑞利,马吉飞,胡烨,路垚,朱蕴暖.耐药型大肠杆菌引发的仔猪大肠杆菌病的PCR快速鉴定[J].天津农学院学报,2014(4):26-28. |
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| 作者姓名： | 王选 赵瑞利 马吉飞 胡烨 路垚 朱蕴暖 |
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| 作者单位： | 天津农学院 动物科学与动物医学学院,天津,300384 |
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| 基金项目： | 天津市应用基础与前沿技术研究计划一般项目“新牛蛙抗菌肽 RGD-嵌合体的分子设计及其靶向抗肿瘤研究”(14JCYBJC-30000)；天津农学院科技发展基金计划项目“用GAP启动子在毕赤酵母中组成型表达牛蛙抗菌肽” |
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| 摘 要： | 利用菌落PCR方法对临床致病菌进行快速鉴定,以天津地区某猪场病死猪胃、肠内容物作为病料进行细菌分离培养,并对所分离的细菌进行药敏试验鉴定。设计16S r RNA通用引物并利用菌落PCR方法扩增所分离细菌的16S r RNA序列,对该序列进行测定和BLAST分析。结果发现,该细菌为一株多重耐药性大肠杆菌。试验建立了仔猪大肠杆菌病的临床快速检测方法。
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| 关 键 词： | 仔猪 耐药性 大肠杆菌 PCR BLAST分析 |
Rapid PCR Identification of Piglet Colibacillosis Caused by Drug Resistant Type Escherichia coli |
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| WANG Xuan,ZHAO Rui-li,MA Ji-fei,HU Ye,LU Yao,ZHU Yun-nuan.Rapid PCR Identification of Piglet Colibacillosis Caused by Drug Resistant Type Escherichia coli[J].Journal of Tianjin Agricultural College,2014(4):26-28. |
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| Authors: | WANG Xuan ZHAO Rui-li MA Ji-fei HU Ye LU Yao ZHU Yun-nuan |
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| Institution: | (College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China ) |
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| Abstract: | In order to investigate the application value of polymerase chain reaction（PCR） technique in detection of pathogenic bacteria in piglets,pigs＇ stomach,intestinal contents as disease materials from a pig farm in Tianjin area were expected to isolate and cultivate the pathogenic bacteria and test the drug sensitivity.Universal primes were designed to detect 16 S r RNA sequences from isolated bacteria by using PCR method and the results were analyzed by the biological software BLAST.The results show that the pathogenic bacteria was a multiple drug resistant type Escherichia coli.A rapid clinical method of detecting colibacillosis of piglet was found in this study. |
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| Keywords: | piglet drug resistance Escherichia coli PCR BLAST analysis |
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