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| VBNC状态鸡白痢沙门氏菌快速诱导条件筛选 | |
| 引用本文： | 胡烨,马吉飞,赵瑞利,边艳,杨久强.VBNC状态鸡白痢沙门氏菌快速诱导条件筛选[J].天津农学院学报,2013(3):37-41. |
| 作者姓名： | 胡烨 马吉飞 赵瑞利 边艳 杨久强 |
| 作者单位： | 1. 天津农学院动物科学系,天津,300384 2. 天津市静海县畜牧水产业发展服务中心,天津,301600 3. 天津市津南区畜牧水产业发展服务中心,天津,300350 |
| 基金项目： | 天津农学院科技发展基金计划项目“VBNC沙门氏菌致病性研究” |
| 摘 要： | 为建立VBNC状态沙门氏菌实验室快速诱导条件，以4℃低温诱导为对照组，在此基础上，添加不同浓度Cu^2＋
和冰醋酸作为快速诱导条件进行试验。结果表明，在4℃低温条件下，添加2-4 mM Cu^2＋对沙门氏菌进入VBNC状态有明显促进作用，与传统诱导条件相比，诱导时间平均提前15d。对诱导进入VBNC状态的沙门氏菌进行16SrRNA鉴定，结果证明，所得菌为VBNC状态沙门氏菌。 |
| 关 键 词： | 活的非可培养状态 沙门氏菌 16S rRNA Cu^2＋ |
Selection of VBNC State Rapid Induction Condition ofSalmonella Pullorum |
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| HU Ye , MA Ji-fei , ZHAO Rui-li , BIAN Yan , YANG Jiu-qiang.Selection of VBNC State Rapid Induction Condition ofSalmonella Pullorum[J].Journal of Tianjin Agricultural College,2013(3):37-41. | |
| Authors: | HU Ye MA Ji-fei ZHAO Rui-li BIAN Yan YANG Jiu-qiang |
| Institution: | 1. Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China; 2. Jinghai Animal Husbandry and Aquaculture Development Service Center of Tianjin City, Tianjin 301600, China; 3. Jinnan Animal Husbandry and Aquaculture Development Service Center of Tianjin City, Tianjin 300350, China) |
| Abstract: | In order to establish the rapid induction condition of VBNC state Salmonella pulomm, the induction at the low temperature of 4 ℃was selected as the control group, and on this basis, the experiment was conducted through adding different concentrations of Cu^2＋ and glacial acetic acid as the rapid induction condition. The results show that adding 2 -4 mM Cu^could obviously promote Salmonella into VBNC state at 4 ℃low temperature, and compared with the traditional induction condition, the induction time could be advanced 15 d averagely. The Salmonella entering into the VBNC state was identified by 16S rRNA test, and the results proved that the stains were Salmonella of VBNC state. |
| Keywords: | viable but non-culturable state Salmonella 16S rRNA Cu2+ |
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