| 紫贻贝配子细胞表面糖基的定位研究 |
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| 引用本文： | 刘慧慧,李太武,苏秀榕.紫贻贝配子细胞表面糖基的定位研究[J].浙江水产学院学报,2011(5):375-380. |
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| 作者姓名： | 刘慧慧 李太武 苏秀榕 |
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| 作者单位： | [1]浙江海洋学院海洋科学学院,浙江舟山316004 [2]宁波大学海洋学院,浙江宁波315211 |
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| 基金项目： | 浙江省自然科学基金资助项目（Y307381）; 国家科技支撑计划（2007BAD43B06） |
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| 摘 要： | 细胞表面糖蛋白中糖的特性对于细胞的相互识别起重要作用,配子细胞表面糖配基的差异恰恰体现不同物种间受精作用的特异性,本研究分别选用可以特异性结合半乳糖、葡萄糖、甘露糖、岩藻糖的FITC标记凝集素,对紫贻贝配子细胞中对应的糖进行研究。结果显示,4种糖在卵细胞边缘的标记特征不明显,仅半乳糖和葡萄糖在一些卵细胞边缘有区域性分布,但在细胞质区,这4种糖均有少量分布;半乳糖和岩藻糖在贻贝的精巢的精细胞和成熟精子区为较均匀的弱阳性标记,甘露糖仅在输精小管边缘有分布,葡萄糖的含量相对较多,但也只是在输精小管边缘以及成熟精子区有一定的标记。上述结果表明,紫贻贝配子细胞表面不同糖基的分布和含量存在一定的差异,造成配子间起到识别作用的糖蛋白组成和结构不同,决定了紫贻贝配子识别的特异性。
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| 关 键 词： | 紫贻贝 配子 糖基 |
Location of Saccharides on the Gamete of Mytilus edulis Linnaeus |
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| Authors: | LIU Hui-hui LI Tai-wu SU Xiu-rong |
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| Institution: | 1.Marine Science School of Zhejiang Ocean University,Zhoushan 316004; 2.Ocean College of Ningbo University,Ningbo 315211,China) |
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| Abstract: | The saccharides of glycoprotein on cell are important for cellular recognition.The differences of sugar ligands on gamete decide specificity of fertilization between interspecies.We selected 4 FITC labeled lectin（bound to mannose,galactose,glucose,fucose） to investgate the saccharides on gamete of Mytilus edulis.Galactose and glucose were labeled regionally on the border of egg,but mannose,galactose,glucose and fucose distributed slightly in cytoplasm.There were weak marks of galactose and fucose in sperm cell and mature sperm.Mannose and glucose distributed the border of spermi duct,but glucose marked apparently in mature sperm.The results showed that the distribution and cotent of diverse saccharides were different,and the construction and structure of glycoprotein were also different.These characters decided the specificity of gamete recognition in M.edulis. |
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| Keywords: | Mytilus edulis gamete saccharide |
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