| 梭鱼和鲻鱼线粒体16S rRNA和COⅠ基因片段的比较分析 |
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| 引用本文： | 程国宝,李三磊,徐冬冬,薛宝贵,耿智,楼宝.梭鱼和鲻鱼线粒体16S rRNA和COⅠ基因片段的比较分析[J].浙江水产学院学报,2012(2):103-106. |
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| 作者姓名： | 程国宝 李三磊 徐冬冬 薛宝贵 耿智 楼宝 |
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| 作者单位： | 浙江海洋学院海洋与渔业研究所,浙江省海洋水产研究所,浙江舟山316100 |
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| 基金项目： | 浙江省科技计划项目（2010F20006） |
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| 摘 要： | 运用PCR技术,扩增了梭鱼和鲻鱼线粒体16S rRNA和COⅠ基因片段,并比较分析了其种间的序列差异。获得16S rRNA基因的540～560 bp碱基序列,出现26个碱基的插入与缺失位点;获得COⅠ基因的602～604 bp碱基序列,出现2个插入缺失位点;16S rRNA和COⅠ基因的序列中G平均含量最低,且（A＋T）含量高于（G＋C）含量,与其他鱼类中的16S rRNA和COⅠ基因片段研究结果相一致。在16S rRNA基因片段中,梭鱼出现1种单倍型,鲻鱼为2种单倍型;在COⅠ基因片段中,梭鱼样品中检测到3个单倍型,鲻鱼样品中检测到5个单倍型。以Takifugu poecilonotus为外群,构建的NJ系统发育树,基于16S rRNA和COⅠ基因序列获得的NJ系统树基本一致,鲻鱼和梭鱼种内个体分别聚为一支,显示16S rRNA和COⅠ基因适合于梭鱼和鲻鱼的物种鉴定。
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| 关 键 词： | 梭鱼 鲻鱼 16SrRNA COⅠ |
Sequence Analysis of 16 S rRNA and CO I Gene Fragment of Liza haematocheila and Mugil cephalus |
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| Institution: | CHENG Guo-bao, LI San-lei, XU Dong-dong, et al (Marine and Fisherery Research Institute of Zhejiang Ocean University, Marine Fisherier Research Institute of Zhejiang Province, Zhoushan 316100, China) |
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| Abstract: | The Ⅰ6S rRNA and cytochrome oxidase subunit I （COⅠ） gene fragments of Liza haematocheila and Mugil cephalus were amplified by PCR, and the sequence variation among interspecies were analyzed. A total of 26 insertion or deletion of sites were detected for 16S rRNA; and 2 insertion or deletion of sites were detected for（CO Ⅰ
） gene. The average content of G was the lowest, and the content of （A＋T） was higher than that of （G＋C）, which was similar to the results reported in other fish species. For 16S rRNA, one haplotype was observed in L. haematocheila and 2 haplotypes were observed in M. cephalus. As for CO Ⅰ gene fragments, 3 haplotypes were detected in L. haematocheila and 5 haplotypes were detected in M. cephalus. Takifugu poe-ble to molecular identification. |
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| Keywords: | Liza haematocheila Mugil cephalus 16S rRNA COⅠ |
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