| 番鸭呼肠孤病毒分子流行病学研究 |
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| 引用本文： | 林锋强,朱小丽,陈少莺,王劭,陈仕龙,程晓霞,江斌,李兆龙.番鸭呼肠孤病毒分子流行病学研究[J].福建农业学报,2012,27(3):217-221. |
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| 作者姓名： | 林锋强 朱小丽 陈少莺 王劭 陈仕龙 程晓霞 江斌 李兆龙 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建福州 350013;福建省畜禽疫病防治工程技术研究中心,福建福州350013 |
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| 基金项目： | 福建省科技计划重大专项，福建省计划项目——省属公益类科研院所基本科研专项，福建省星火科技计划项目 |
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| 摘 要： | 选取1998～2009年福建省不同地区番鸭中分离到番鸭呼肠孤病毒分离株中具有代表性的10株,用RT-PCR技术扩增其S1和S4基因的功能区片段,进行序列测定.序列分析表明所扩增的S1基因的目的片段为819bp,S4基因目的片段为992 bp.参照国内外已发表的部分毒株S1和S4基因序列,构建番鸭呼肠孤病毒的遗传进化树,分析遗传进化关系.国内各分离株S1基因同源性为92.2％～96.7％,与国外89026株同源性为88.5％～92.8％,与禽呼肠孤病毒S1133株同源性为76％～78％.国内各分离株S4基因同源性为95.2％～99.3％,与国外89026株同源性为92.3％～94.5％,与禽呼肠孤病毒S1133株同源性为21.2％～21.5％.通过遗传进化树可以看到MDRV与同属的ARV序列形成了2个大分支,而MDRV序列分为2个小分支:一支为10株分离株和国内分离株,说明国内分离株没有明显差异;一支为国外分离株89026株,这说明国内分离株与国外毒株存在地域差异.
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| 关 键 词： | 番鸭呼肠孤病毒 S基因 分子流行病学 |
Molecular Epidemiology of Muscovy Duck Reovirus |
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| LIN Feng-qiang
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ZHU Xiao-li
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CHEN Shao-ying
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WANG Shao
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CHEN Shi-long
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CHENG Xiao-xia
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JIANG Bin
,
LI Zhao-long.Molecular Epidemiology of Muscovy Duck Reovirus[J].Fujian Journal of Agricultural Sciences,2012,27(3):217-221. |
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| Authors: | LIN Feng-qiang ZHU Xiao-li CHEN Shao-ying WANG Shao CHEN Shi-long CHENG Xiao-xia JIANG Bin LI Zhao-long |
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| Institution: | 1,2 (1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences, Fuzhou,Fujian 350013,China;2.Fujian Animal Diseases Control Technology Development Center,Fuzhou,Fujian 350013,China) |
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| Abstract: | The primers were designed to amplify the S1 and S4 gene according to the muscovy duck reovirus(MDRV) sequencein Genbank.S1 and S4 gene of MDRV strains isolated in China were cloned and then sequenced and analyzed.The results of showed S4 and S1 gene was 992 bp and 819 bp in length,respectively;the nucleotide sequence of S1 segment shared 92.2%-96.7% identity in China strains,but 92.2%-96.7% identity with France strain,89026;and 76%-78% identity with ARV strain,S1133.The nucleotide suquence of S4 segment shared 95.2%-99.3% idnentity in China stains,but 92.3%-94.5% identity with France strain,89026,and 21.2%-21.5% identity with ARV strain,S1133.Phylogenetic analysis demonstrated that MDRV was quite different from ARV.The Ten MDRV strains isolated in China has no distinct difference,but they were different from 89026(isolated in France).The study provide the basic research on the prevention with muscovy duck reovirusis. |
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| Keywords: | muscovy duck reovirus S class genome molecular epidemiology |
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