| 源自猪场环境大肠杆菌耐药性检测 及其产ESBLs菌耐药基因特征 |
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| 引用本文： | 郑新添,黄其春,陈星星,张孝辉,谢建斌.源自猪场环境大肠杆菌耐药性检测 及其产ESBLs菌耐药基因特征[J].安徽农业大学学报,2014,41(3):375. |
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| 作者姓名： | 郑新添 黄其春 陈星星 张孝辉 谢建斌 |
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| 作者单位： | 龙岩学院福建省预防兽医学与兽医生物技术重点实验室; |
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| 基金项目： | 福建省教育厅B类项目(JBS10154);福建省科技厅区域科技重大项目(2009N3013)共同资助 |
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| 摘 要： | 为了解猪场周边水和土壤环境中大肠杆菌耐药性及其中产ESBLs菌的耐药基因特征,用纸片扩散法检测了从福建省龙岩市5个规模化猪场周边水和土壤中分离的55株大肠杆菌对12种抗菌药的耐药性,并对其中产ESBLs菌进行了表型筛选;在此基础上,用CTX-M、TEM、SHV及OXA等4种ESBLs基因引物进行PCR检测与分析。结果表明,猪场环境分离的大肠杆菌对青霉素类、环丙沙星、四环素等耐药率较高,其中水源菌耐药率较高。55株大肠杆菌中,19株检出ESBLs基因,ESBLs检出率为34.5%。ESBLs基因型检测结果显示,CTX-M、SHV及TEM的检出率分别为23.6%(13/55),1.8%(1/55)和9.1%(5/55),未检出OXA基因。本试验结果表明,龙岩市猪场环境大肠杆菌对选用抗菌素耐药率较高,其产ESBLs菌的主要ESBLs基因型为CTX-M。
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| 关 键 词： | 猪场 环境 大肠杆菌 ESBLs 耐药性 |
| 收稿时间： | 2013/11/22 0:00:00 |
Antimicrobial susceptibility and ESBLs' genotype of Escherichia coli isolated from external environment nearby pig farm |
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| ZHENG Xintian,HUANG Qichun,CHEN Xingxing,ZHANG Xiaohui and XIE Jianbin.Antimicrobial susceptibility and ESBLs' genotype of Escherichia coli isolated from external environment nearby pig farm[J].Journal of Anhui Agricultural University,2014,41(3):375. |
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| Authors: | ZHENG Xintian HUANG Qichun CHEN Xingxing ZHANG Xiaohui and XIE Jianbin |
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| Institution: | Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology of Fujian Province, Longyan University, Longyan 364012,Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology of Fujian Province, Longyan University, Longyan 364012,Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology of Fujian Province, Longyan University, Longyan 364012,Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology of Fujian Province, Longyan University, Longyan 364012 and Key Laboratory of Preventive Veterinary Medicine and Veterinary Biotechnology of Fujian Province, Longyan University, Longyan 364012 |
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| Abstract: | |
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| Keywords: | pig farm environment Escherichia coli ESBLs antimicrobial susceptibility |
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