| 茶树EST SSRs特点及应用 |
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| 引用本文： | 刘本英,孙雪梅,蒋会兵,李友勇,宋维希,马 玲,矣 兵,汪云刚,王平盛.茶树EST SSRs特点及应用[J].西北农业学报,2011,20(3):161-167. |
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| 作者姓名： | 刘本英 孙雪梅 蒋会兵 李友勇 宋维希 马 玲 矣 兵 汪云刚 王平盛 |
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| 作者单位： | 云南省农业科学院茶叶研究所,云南动海,666201 |
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| 基金项目： | 云南省农科院茶叶所所列项目(2009A0937); 国家现代农业产业技术体系(茶叶)建设项目; 云南省自然科学基金重点项目(2006C0012Z) |
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| 摘 要： | 利用NCBI公共数据库下载获得的3 306条茶树EST,去除其中低质量和冗余的序列,得到全长为635.46 kb的1 335条无冗余EST,在这些序列中共发现216个SSR,分布于185个EST中,出现的频率为16.18%,平均每2.94 kb的表达序列中有1个SSR。茶树的EST-SSR类型丰富,其中二核苷酸重复出现的频率最高,占主导地位。利用Primer 5.0软件从185条含有SSR的EST中设计引物100对,从中随机选取60对引物检测EST-SSR在云南茶树资源中的多态性。结果表明,有30对引物在供试的63份茶树资源中得到有效扩增,并具有多态性;共检测到等位基因数71个,每对引物检测出的等位基因数2~8个,平均4.9个;多态信息量(PIC)变幅为0~0.731,平均为0.499;观察杂合度变幅为0~0.970,平均0.392。EST-SSR标记分析表明云南茶树资源具有较高的遗传多样性。
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| 关 键 词： | 茶树 云南 EST-SSRs 多态性 |
Characterization and Application of EST-SSR Markers in Tea Plants(Camellia sinensis(L.)O.Kuntze) |
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| LIU Benying,SUN Xuemei,JIANG Huibing,LI Youyong,SONG Weixi,MA Ling,YI Bing,WANG Yungang and WANG Pingsheng.Characterization and Application of EST-SSR Markers in Tea Plants(Camellia sinensis(L.)O.Kuntze)[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(3):161-167. |
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| Authors: | LIU Benying SUN Xuemei JIANG Huibing LI Youyong SONG Weixi MA Ling YI Bing WANG Yungang and WANG Pingsheng |
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| Institution: | LIU Benying,SUN Xuemei,JIANG Huibing,LI Youyong,SONG Weixi,MA Ling,YI Bing,WANG Yungang and WANG Pingsheng (Tea Research Institute of Yunnan Academy of Agricultural Science,Menghai Yunnan 666201,China) |
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| Abstract: | The frequency and characteristics of SSR were analyzed and clarified in ESTs of tea plant(Camellia sinensis(L.) O.Kuntze).3 306 ESTs of tea plant were downloaded from the public database of NCBI,among which the redundant or low quality were removed,finally obtained 1335 non-redundant ESTs with total length of 635.46 kb.Totally 216 SSRs distributed in 185 ESTs were found,accounting for 16.18% of the non-redundant ESTs,and the average distance of distribution is 1/2.94 kb.EST-SSR types in tea plant are consid... |
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| Keywords: | Camellia sinensis(L )O Kuntze Yunnan EST-SSRs Polymorphic traits |
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