| 甘肃部分地区致羔羊腹泻大肠埃希菌的分离鉴定及耐药性分析 |
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| 引用本文： | 妥 鑫,王胜义,王 慧,崔东安,黄美州,刘永明.甘肃部分地区致羔羊腹泻大肠埃希菌的分离鉴定及耐药性分析[J].西北农业学报,2017(2):167-172. |
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| 作者姓名： | 妥 鑫 王胜义 王 慧 崔东安 黄美州 刘永明 |
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| 作者单位： | （中国农业科学院 兰州畜牧与兽药研究所，农业部兽用药物创制重点实验室，甘肃省新兽药工程重点实验室，甘肃省中兽药工程技术研究中心，兰州 730050） |
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| 基金项目： | 公益性行业专项课题（20130304-17）；国家科技支撑计划（2012BAD12B03）。 |
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| 摘 要： | 为调查甘肃地区大肠埃希菌对新生羔羊腹泻的致病性及其耐药性,从甘肃省8个养殖场采集新生腹泻羔羊粪便92份。采用16S rDNA扩增结合微生物鉴别培养技术对病料中的致病性大肠埃希菌进行鉴定,随机选取分离的6株菌株进行致病性试验,并采用Kirby-Bauer纸片琼脂扩散法对分离得到的菌株进行耐药性分析。结果表明,从92份病料中分离得到21株致病性大肠埃希菌,选取的6株菌均有一定的致病性,耐药性分析结果显示该地的大肠埃希菌对羊场常用的多种抗生素耐药。表明大肠埃希菌仍是引起甘肃地区新生羔羊腹泻的主要病原菌,而且耐药性严重。
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| 关 键 词： | 新生羔羊 腹泻 大肠埃希菌 致病性 耐药性 |
Identification and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheic Neonatal Lambs in Gansu Area |
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| Abstract: | The objective of the study was to research the pathogenesis and drug resistance of Escherichia coli in diarrheic neonatal lambs in Gansu area. The 92 feces samples were collected from 8 lamb farms in Gansu province, where the lambs appeared diarrhea symptoms. The pathogenic E.coli was isolated and identified using amplification technology of 16S rDNA and microbial cultivation methods and 6 strains were selected randomly to conduct pathogenicity and drug resistance test. The results showed that 21 pathogenic E.coli strains were isolated from 92 samples; the 6 strains selected randomly possessed of certain pathogenicity; and these pathogenic E.coli in Gansu area had appeared high resistance to commonly used antimicrobial drugs. The results suggested that the E.coli was still the etiology of the diarrheic neonatal lambs in Gansu area and with very serious drug resistance. |
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| Keywords: | Neonatal Diarrhea E coli Pathogenicity Drug resistance |
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