| 大酸枣与酸枣、枣亲缘关系的RAPD分析 |
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| 引用本文： | 刘冬芝,时 燕,赵登超,王 雪,侯立群.大酸枣与酸枣、枣亲缘关系的RAPD分析[J].西北农业学报,2010,19(4):160-164. |
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| 作者姓名： | 刘冬芝 时 燕 赵登超 王 雪 侯立群 |
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| 作者单位： | 1. 山东师范大学,生命科学学院,济南,250014
2. 山东省林业科学研究院,济南,250014 |
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| 摘 要： | 利用RAPD分子标记技术对大酸枣、酸枣及枣等11份种质材料进行了亲缘关系和遗传多样性检测,并采用离差平方和法进行聚类分析。结果表明,16条引物共获得115条谱带,其中多态性带84条,占总扩增谱带的73.04%。表明RAPD技术能较好地用于大酸枣、酸枣及枣亲缘关系的研究。从聚类分析图得,在等值线λ=15处,可将供试材料分为3类群:第1类包括无核小枣、金丝小枣、圆铃枣、冬枣4个枣品种;第2类包括大酸枣、s4、s5;第3类是由s1、s2s、3组成的酸枣群;表明大酸枣的分类地位处于供试酸枣和枣品种之间,推测大酸枣可能为供试酸枣与枣品种的中间类型。从欧氏距离上看出,大酸枣与供试酸枣的平均欧氏距离大于与枣品种间的平均欧氏距离,表明大酸枣与供试枣品种间亲缘关系较近。
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| 关 键 词： | 枣 酸枣 遗传多样性 亲缘关系 聚类分析 |
RAPD Analysis on Relationship of Dasuanzao,Ziziphus spinosa and Ziziphus jujuba |
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| LIU Dongzhi,SHI Yan,ZHAO Dengchao,WANG Xue and HOU Liqun.RAPD Analysis on Relationship of Dasuanzao,Ziziphus spinosa and Ziziphus jujuba[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(4):160-164. |
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| Authors: | LIU Dongzhi SHI Yan ZHAO Dengchao WANG Xue and HOU Liqun |
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| Institution: | LIU Dongzhi1,SHI Yan1,ZHAO Dengchao2,WANG Xue1 , HOU Liqun2(1.College of Life Science,Sh,ong Normal University,Jinan 250014,China,2.Sh,ong Academy of Forestry Science,China) |
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| Abstract: | The genetic diversity and systematic relationship between Dasuanzao,Ziziphus spinosa and Ziziphus jujuba were studied using RAPD(random amplified polymorphic DNA) technique.A tree diagram was constructed by using Ward method.Totally,115 DNA fragments were amplified with 16 random primers.The polymorphic DNA fragments were 84,accounting for 73.04 % of 115 fragments.It was shown that RAPD could be used to analysis genetic relationship among Dasuanzao,Ziziphus spinosa and Ziziphus jujuba.Cluster analysis showe... |
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| Keywords: | RAPD |
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