| 玉米中过表达 ZmMRP-1基因的遗传转化与产量鉴定 |
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| 引用本文： | 金如昌,杜邓襄,李旭欣,杨 涵,刘江江,郭金洁,张 丹,张方东.玉米中过表达 ZmMRP-1基因的遗传转化与产量鉴定[J].西北农业学报,2020,30(11):1638-1641. |
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| 作者姓名： | 金如昌 杜邓襄 李旭欣 杨 涵 刘江江 郭金洁 张 丹 张方东 |
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| 作者单位： | （华中农业大学 植物科学技术学院， 武汉 430070） |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2016ZX08003003)。 |
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| 摘 要： | 构建植物过表达载体pHZM1N-P ZmMRP-1::ZmMRP-1，利用农杆菌介导的玉米愈伤组织转化法将其导入玉米自交系A188中，获得12个独立的PCR阳性转化株系。对转基因株系进行Southern blot检测、qRT-PCR分析，发现OE-8、OE-19株系以单拷贝形式插入玉米基因组中并过量表达。对获得的转 ZmMRP-1基因T2代材料进行玉米产量相关性状考察，相比较野生型，转基因株系在粒长、粒宽、百粒质量等方面均显著提高，可有效改良玉米产量相关性状，为高产转基因玉米新品种培育提供基础材料支持。
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| 关 键 词： | 玉米 ZmMRP-1基因 遗传转化 过表达 产量性状 |
Genetic Transformation and Yield Identification of Overexpressed ZmMRP-1 Gene in Maize |
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| JIN Ruchang,DU Dengxiang,LI Xuxin,YANG Han,LIU Jiangjiang,GUO Jinjie,ZHANG Dan and ZHANG Fangdong.Genetic Transformation and Yield Identification of Overexpressed ZmMRP-1 Gene in Maize[J].Acta Agriculturae Boreali-occidentalis Sinica,2020,30(11):1638-1641. |
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| Authors: | JIN Ruchang DU Dengxiang LI Xuxin YANG Han LIU Jiangjiang GUO Jinjie ZHANG Dan and ZHANG Fangdong |
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| Abstract: | In this paper, the plant overexpression vector pHZM1N-P ZmMRP-1::ZmMRP-1 was constructed and introduced into the maize inbred line A188 by using Agrobacterium-mediated maize callus transformation, from which 12 independent PCR-positive transformed lines were obtained.Southern blot and qRT-PCR analysis of the transgenic lines revealed that the ZmMRP-1 gene was inserted into the maize genome as a single copy and overexpressed in the OE-8 and OE-19 lines.Comparing the yield-related parameters of the T2 generation materials of the transgenic ZmMRP-1 with wild type lines, the results showed that the transgenic lines had significantly improved grain length, grain width, and 100-grain mass.In this study, the yield-related traits of maize were effectively improved, which provide the basic material for the cultivation of new high-yield genetically modified maize cultivars. |
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